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Literature summary for 6.1.1.6 extracted from

  • Stello, T.; Hong, M.; Musier-Forsyth, K.
    Efficient aminoacylation of tRNALys,3 by human lysyl-tRNA synthetase is dependent on covalent continuity between the acceptor stem and the anticodon domain (1999), Nucleic Acids Res., 27, 4823-4829.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
human tRNALys anticodon domain from wild-type tRNALys, N-terminally truncated mutant enzyme Homo sapiens
human tRNALys,3'-14mer mutant
-
Homo sapiens
human tRNALys,3'-17mer mutant
-
Homo sapiens
human tRNALys,3'-7mer mutant
-
Homo sapiens
human tRNALys-3'oxidized 3'-oxidized by periodate, N-terminally truncated mutant enzyme Homo sapiens
human tRNALysU35G anticodon domain N-terminally truncated mutant enzyme Homo sapiens
human tRNALysU35G mutant defective substrate in both binding and catalysis, N-terminally truncated mutant enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0034
-
tRNALys human wild-type tRNALys, 30°C, wild-type and N-terminally truncated mutant enzymes Homo sapiens
0.0037
-
tRNALysU36C human mutant tRNALys, 30°C Homo sapiens
0.0037
-
tRNALys human wild-type tRNALys, 20°C, wild-type enzyme Homo sapiens
0.0078
-
tRNALysU35C human mutant tRNALys, 30°C Homo sapiens
0.0095
-
tRNALysU36A human mutant tRNALys, 30°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + lysine + tRNALys Homo sapiens
-
AMP + L-lysyl-tRNALys + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
His-tagged recombinant wild-type purified from Escherichia coli strain DE3, N-terminally truncated recombinant enzyme, comprising amino acid residues 66-597, purified from enzyme-deficient variant Escherichia coli strain DE3
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + lysine + tRNALys
-
Homo sapiens AMP + L-lysyl-tRNALys + diphosphate
-
?
ATP + lysine + tRNALys human tRNALys wild-type Homo sapiens AMP + L-lysyl-tRNALys + diphosphate
-
?
ATP + lysine + tRNALys,3'-59mer human tRNALys mutant Homo sapiens AMP + L-lysyl-tRNALys, 3'-59mer + diphosphate
-
?
ATP + lysine + tRNALysU35A human tRNALys mutant, from in vitro translation, very low activity Homo sapiens AMP + L-lysyl-tRNALysU35A + diphosphate
-
?
ATP + lysine + tRNALysU35C human tRNALys mutant, from in vitro translation, 153fold decreased activity compared to the wild-type Homo sapiens AMP + L-lysyl-tRNALysU35C + diphosphate
-
?
ATP + lysine + tRNALysU36A human tRNALys mutant, from in vitro translation, 182fold decreased activity compared to the wild-type Homo sapiens AMP + L-lysyl-tRNALysU36A + diphosphate
-
?
ATP + lysine + tRNALysU36C human tRNALys mutant, from in vitro translation, 11fold decreased activity compared to the wild-type Homo sapiens AMP + L-lysyl-tRNALysU36C + diphosphate
-
?
additional information substrate specificity, no activity with human tRNALysU35G mutant Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
L-Lysine-transfer RNA ligase
-
Homo sapiens
Lysine translase
-
Homo sapiens
Lysine--tRNA ligase
-
Homo sapiens
Lysine-tRNA synthetase
-
Homo sapiens
LysRS
-
Homo sapiens
Lysyl-transfer ribonucleate synthetase
-
Homo sapiens
Lysyl-transfer RNA synthetase
-
Homo sapiens
Lysyl-tRNA synthetase
-
Homo sapiens
Synthetase, lysyl-transfer ribonucleate
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00026
-
tRNALys,3'-59mer human tRNALys mutant + human tRNALys,3'-7mer mutant, 20°C, wild-type enzyme Homo sapiens
0.0068
-
tRNALysU36A human mutant tRNALys, 30°C, N-terminally truncated mutant enzyme Homo sapiens
0.0079
-
tRNALysU35C human mutant tRNALys, 30°C, N-terminally truncated mutant enzyme Homo sapiens
0.046
-
tRNALysU36C human mutant tRNALys, 30°C, N-terminally truncated mutant enzyme Homo sapiens
0.38
-
tRNALys human wild-type tRNALys, 30°C, N-terminally truncated mutant enzyme Homo sapiens
0.88
-
tRNALys,3'-59mer human tRNALys mutant + human tRNALys,3'-14mer mutant, 20°C, wild-type enzyme Homo sapiens
1.5
-
tRNALys,3'-59mer human tRNALys mutant + human tRNALys,3'-17mer mutant, 20°C, wild-type enzyme Homo sapiens
1.7
-
tRNALys human wild-type tRNALys, 20°C, wild-type enzyme Homo sapiens
2
-
tRNALys human wild-type tRNALys, 30°C, wild-type enzyme Homo sapiens
6.08
-
tRNALys,3'-59mer human tRNALys mutant + human tRNALys,3'-14mer mutant, 20°C, wild-type enzyme Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0013
-
human tRNALys-3'oxidized 3'-oxidized by periodate, 30°C, N-terminally truncated mutant enzyme Homo sapiens
0.005
-
human tRNALys anticodon domain 30°C, N-terminally truncated mutant enzyme Homo sapiens
0.005
-
human tRNALysU35G anticodon domain 30°C, N-terminally truncated mutant enzyme Homo sapiens
0.042
-
human tRNALysU35G mutant 30°C, N-terminally truncated mutant enzyme Homo sapiens