Inhibitors | Comment | Organism | Structure |
---|---|---|---|
human tRNALys anticodon domain | from wild-type tRNALys, N-terminally truncated mutant enzyme | Homo sapiens | |
human tRNALys,3'-14mer mutant | - |
Homo sapiens | |
human tRNALys,3'-17mer mutant | - |
Homo sapiens | |
human tRNALys,3'-7mer mutant | - |
Homo sapiens | |
human tRNALys-3'oxidized | 3'-oxidized by periodate, N-terminally truncated mutant enzyme | Homo sapiens | |
human tRNALysU35G anticodon domain | N-terminally truncated mutant enzyme | Homo sapiens | |
human tRNALysU35G mutant | defective substrate in both binding and catalysis, N-terminally truncated mutant enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0034 | - |
tRNALys | human wild-type tRNALys, 30°C, wild-type and N-terminally truncated mutant enzymes | Homo sapiens | |
0.0037 | - |
tRNALysU36C | human mutant tRNALys, 30°C | Homo sapiens | |
0.0037 | - |
tRNALys | human wild-type tRNALys, 20°C, wild-type enzyme | Homo sapiens | |
0.0078 | - |
tRNALysU35C | human mutant tRNALys, 30°C | Homo sapiens | |
0.0095 | - |
tRNALysU36A | human mutant tRNALys, 30°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + lysine + tRNALys | Homo sapiens | - |
AMP + L-lysyl-tRNALys + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
His-tagged recombinant wild-type purified from Escherichia coli strain DE3, N-terminally truncated recombinant enzyme, comprising amino acid residues 66-597, purified from enzyme-deficient variant Escherichia coli strain DE3 | - |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + lysine + tRNALys | - |
Homo sapiens | AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
ATP + lysine + tRNALys | human tRNALys wild-type | Homo sapiens | AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
ATP + lysine + tRNALys,3'-59mer | human tRNALys mutant | Homo sapiens | AMP + L-lysyl-tRNALys, 3'-59mer + diphosphate | - |
? | |
ATP + lysine + tRNALysU35A | human tRNALys mutant, from in vitro translation, very low activity | Homo sapiens | AMP + L-lysyl-tRNALysU35A + diphosphate | - |
? | |
ATP + lysine + tRNALysU35C | human tRNALys mutant, from in vitro translation, 153fold decreased activity compared to the wild-type | Homo sapiens | AMP + L-lysyl-tRNALysU35C + diphosphate | - |
? | |
ATP + lysine + tRNALysU36A | human tRNALys mutant, from in vitro translation, 182fold decreased activity compared to the wild-type | Homo sapiens | AMP + L-lysyl-tRNALysU36A + diphosphate | - |
? | |
ATP + lysine + tRNALysU36C | human tRNALys mutant, from in vitro translation, 11fold decreased activity compared to the wild-type | Homo sapiens | AMP + L-lysyl-tRNALysU36C + diphosphate | - |
? | |
additional information | substrate specificity, no activity with human tRNALysU35G mutant | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-Lysine-transfer RNA ligase | - |
Homo sapiens |
Lysine translase | - |
Homo sapiens |
Lysine--tRNA ligase | - |
Homo sapiens |
Lysine-tRNA synthetase | - |
Homo sapiens |
LysRS | - |
Homo sapiens |
Lysyl-transfer ribonucleate synthetase | - |
Homo sapiens |
Lysyl-transfer RNA synthetase | - |
Homo sapiens |
Lysyl-tRNA synthetase | - |
Homo sapiens |
Synthetase, lysyl-transfer ribonucleate | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00026 | - |
tRNALys,3'-59mer | human tRNALys mutant + human tRNALys,3'-7mer mutant, 20°C, wild-type enzyme | Homo sapiens | |
0.0068 | - |
tRNALysU36A | human mutant tRNALys, 30°C, N-terminally truncated mutant enzyme | Homo sapiens | |
0.0079 | - |
tRNALysU35C | human mutant tRNALys, 30°C, N-terminally truncated mutant enzyme | Homo sapiens | |
0.046 | - |
tRNALysU36C | human mutant tRNALys, 30°C, N-terminally truncated mutant enzyme | Homo sapiens | |
0.38 | - |
tRNALys | human wild-type tRNALys, 30°C, N-terminally truncated mutant enzyme | Homo sapiens | |
0.88 | - |
tRNALys,3'-59mer | human tRNALys mutant + human tRNALys,3'-14mer mutant, 20°C, wild-type enzyme | Homo sapiens | |
1.5 | - |
tRNALys,3'-59mer | human tRNALys mutant + human tRNALys,3'-17mer mutant, 20°C, wild-type enzyme | Homo sapiens | |
1.7 | - |
tRNALys | human wild-type tRNALys, 20°C, wild-type enzyme | Homo sapiens | |
2 | - |
tRNALys | human wild-type tRNALys, 30°C, wild-type enzyme | Homo sapiens | |
6.08 | - |
tRNALys,3'-59mer | human tRNALys mutant + human tRNALys,3'-14mer mutant, 20°C, wild-type enzyme | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
human tRNALys-3'oxidized | 3'-oxidized by periodate, 30°C, N-terminally truncated mutant enzyme | Homo sapiens | |
0.005 | - |
human tRNALys anticodon domain | 30°C, N-terminally truncated mutant enzyme | Homo sapiens | |
0.005 | - |
human tRNALysU35G anticodon domain | 30°C, N-terminally truncated mutant enzyme | Homo sapiens | |
0.042 | - |
human tRNALysU35G mutant | 30°C, N-terminally truncated mutant enzyme | Homo sapiens |