Cloned (Comment) | Organism |
---|---|
recombinant expression | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
unliganded enzyme and in complex with L-lysine, vapor diffusion hanging drop method, protein solution: 20 mg/ml protein, 20 mM Tris-HCl, pH 8.0, 10 mM 2-mercaptoethanol, 10 mM MgCl2, plus equal volume of reservoir solution: 0.1 M HEPES, pH 7.5, 46-50% saturated ammonium sulfate solution, 2-4% polyethylene glycol 400, 15-20% glycerol, 4-18°C, X-ray difraction structure determination at 2.7 A resolution, and analysis | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
2 * 58000, recombinant enzyme, crystal structure | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-lysine + tRNALys | Escherichia coli | - |
AMP + L-lysyl-tRNALys + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A8N3 | class II enzyme | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-lysine + tRNALys = AMP + diphosphate + L-lysyl-tRNALys | active site structure and substrate binding | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-lysine + tRNALys | - |
Escherichia coli | AMP + L-lysyl-tRNALys + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 58000, recombinant enzyme, crystal structure | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
L-Lysine-transfer RNA ligase | - |
Escherichia coli |
Lysine translase | - |
Escherichia coli |
Lysine--tRNA ligase | - |
Escherichia coli |
Lysine-tRNA synthetase | - |
Escherichia coli |
LysRS | - |
Escherichia coli |
Lysyl-transfer ribonucleate synthetase | - |
Escherichia coli |
Lysyl-transfer RNA synthetase | - |
Escherichia coli |
Lysyl-tRNA synthetase | - |
Escherichia coli |
Synthetase, lysyl-transfer ribonucleate | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |