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Literature summary for 6.1.1.6 extracted from

  • Hele, P.; Barber, R.
    Lysyl tRNA synthetase of Escherichia coli. Formation and reactions of ATP-enzyme and lysyl-AMP-enzyme complexes (1972), Biochim. Biophys. Acta, 258, 319-331.
    View publication on PubMed
Search for more literature for 6.1.1.6

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ synthesis of lysyl-tRNA from lysyl-AMP-enzyme inhibited, formation of Lys-tRNALys from lysine, tRNA and ATP-enzyme complex or ATP is dependent on Mg2+ Escherichia coli
NH4+ formation of Lys-tRNALys from lysine, tRNA and ATP-enzyme complex or ATP Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 Lys aminoacylation Escherichia coli
0.005
-
 L-Lys aminoacylation, enzyme form EII Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ formation of Lys-tRNALys from lysine, tRNA and ATP-enzyme complex or ATP is dependent on Mg2+ Escherichia coli
NH4+ synthesis of lysyl-tRNA from lysyl-AMP-enzyme is dependent on NH4+ or other monovalent cations Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
 1 * 72000 + 1 + 62000, SDS-PAGE Escherichia coli
150000
-
 gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 B
-
Escherichia coli B / ATCC 11303
-
 B
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19.6
-
 ATP-diphosphate exchange Escherichia coli

Storage Stability

Storage Stability Organism
-15°C, 2 years Escherichia coli
4°C, 8 months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + lysine + tRNALys
-
 Escherichia coli AMP + L-lysyl-tRNALys + diphosphate
-
 ?
ATP + lysine + tRNALys
-
 Escherichia coli B / ATCC 11303 AMP + L-lysyl-tRNALys + diphosphate
-
 ?
additional information lysine-dependent ATP-diphosphate exchange reaction: lysine + ATP + enzyme/lysine-AMP-enzyme + diphosphate Escherichia coli ?
-
 ?
additional information lysine-dependent ATP-diphosphate exchange reaction: lysine + ATP + enzyme/lysine-AMP-enzyme + diphosphate Escherichia coli B / ATCC 11303 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 72000 + 1 + 62000, SDS-PAGE Escherichia coli