Any feedback?
Literature summary for 6.1.1.6 extracted from
- Lysyl-tRNA synthetase from Bacillus stearothermophilus. Purification and fluorometric and kinetic analysis of the binding of substrates, L-lysine and ATP (1996), J. Biochem., 119, 680-689.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 6-amino-n-hexanoic acid | ATP-diphosphate exchange reaction | Geobacillus stearothermophilus |  |
| adenosine | - |
Geobacillus stearothermophilus | |
| ATP | substrate inhibition in the aminoacylation reaction above 0.4 mM, no inhibition in the ATP-diphosphate exchange reaction | Geobacillus stearothermophilus | |
| cadaverine | ATP-diphosphate exchange reaction | Geobacillus stearothermophilus | |
| L-Lysine amide | ATP-diphosphate exchange reaction | Geobacillus stearothermophilus | |
| L-Lysine hydroxamate | ATP-diphosphate exchange reaction | Geobacillus stearothermophilus | |
| Nepsilon-Acetyl-L-lysine | ATP-diphosphate exchange reaction | Geobacillus stearothermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0164 | - |
L-Lys | aminoacylation | Geobacillus stearothermophilus | |
| 0.0232 | - |
ATP | aminoacylation | Geobacillus stearothermophilus | |
| 0.0236 | - |
L-Lys | ATP-diphosphate exchange | Geobacillus stearothermophilus | |
| 0.0651 | - |
ATP | ATP-diphosphate exchange | Geobacillus stearothermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57700 | - |
2 * 57700, homodimer, SDS-PAGE, amino acid composition | Geobacillus stearothermophilus |
| 120000 | - |
gel filtration | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
NCA1503 | - |
| Geobacillus stearothermophilus NCA1503 | - |
NCA1503 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + L-lysine + tRNALys = AMP + diphosphate + L-lysyl-tRNALys | sequential ordered mechanism | Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.79 | - |
- |
Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-lysine + tRNALys | - |
Geobacillus stearothermophilus | AMP + diphosphate + L-lysyl-tRNALys | - |
r | |
| ATP + L-lysine + tRNALys | - |
Geobacillus stearothermophilus NCA1503 | AMP + diphosphate + L-lysyl-tRNALys | - |
r | |
| ATP + lysine + tRNALys | - |
Geobacillus stearothermophilus | AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
| ATP + lysine + tRNALys | - |
Geobacillus stearothermophilus NCA1503 | AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
| additional information | lysine-dependent ATP-diphosphate exchange reaction: lysine + ATP + enzyme/lysine-AMP-enzyme + diphosphate | Geobacillus stearothermophilus | ? | - |
? | |
| additional information | L-lysine-dependent synthesis of 5',5'-diadenosine tetraphosphate (Ap4A) | Geobacillus stearothermophilus | ? | - |
? | |
| additional information | lysine-dependent ATP-diphosphate exchange reaction: lysine + ATP + enzyme/lysine-AMP-enzyme + diphosphate | Geobacillus stearothermophilus NCA1503 | ? | - |
? | |
| additional information | L-lysine-dependent synthesis of 5',5'-diadenosine tetraphosphate (Ap4A) | Geobacillus stearothermophilus NCA1503 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 57700, homodimer, SDS-PAGE, amino acid composition | Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
aminoacylation reaction and ATP-diphosphate exchange, pH 8 | Geobacillus stearothermophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
inactive at 70°C | Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable below, pH 8.0, 5 h | Geobacillus stearothermophilus |
| 60 | - |
after 4 h complete loss of aminoacylation activity, 3 min, stable in presence of all substrates except lysine | Geobacillus stearothermophilus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.2 | - |
L-lysine | - |
Geobacillus stearothermophilus | |
| 40.9 | - |
ATP | ATP-diphosphate exchange | Geobacillus stearothermophilus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
- |
Geobacillus stearothermophilus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
5 h, stable below 50°C | Geobacillus stearothermophilus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
