Cloned (Comment) | Organism |
---|---|
gene ileS2, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strains DH5alpha and Ts331 mediates resistance against pseudomonic acid, complementation of the ileS-deficient strain Ts331 | Pseudomonas fluorescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition of isozyme IleRS-R2 by pseudomonic acid, i.e. muciproin | Pseudomonas fluorescens | |
pseudomonic acid | i.e. muciproin | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00125 | - |
L-isoleucine | pH 7.5, recombinant isozyme IleRS-R1 | Pseudomonas fluorescens | |
0.007 | - |
ATP | pH 7.5, recombinant isozyme IleRS-R1 | Pseudomonas fluorescens | |
0.01 | - |
ATP | pH 7.5, native isozyme IleRS-R2 | Pseudomonas fluorescens | |
0.012 | - |
L-isoleucine | pH 7.5, recombinant isozyme IleRS-R2 | Pseudomonas fluorescens | |
0.285 | - |
ATP | pH 7.5, recombinant isozyme IleRS-R2 | Pseudomonas fluorescens | |
0.33 | - |
ATP | pH 7.5, native isozyme IleRS-R2 | Pseudomonas fluorescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-isoleucine + tRNAIle | Pseudomonas fluorescens | - |
AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? | |
ATP + L-isoleucine + tRNAIle | Pseudomonas fluorescens NCIB 10586 | - |
AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
gene ileS1, pseudomonic acid-sensitive isozyme IleRS-R1, recombinant enzyme expressed in Escherichia coli | - |
Pseudomonas fluorescens | Q8L1B1 | pseudomonic acid-resistant isozyme IleRS-R2, gene ileS2 | - |
Pseudomonas fluorescens NCIB 10586 | - |
gene ileS1, pseudomonic acid-sensitive isozyme IleRS-R1, recombinant enzyme expressed in Escherichia coli | - |
Pseudomonas fluorescens NCIB 10586 | Q8L1B1 | pseudomonic acid-resistant isozyme IleRS-R2, gene ileS2 | - |
Purification (Comment) | Organism |
---|---|
native enzyme, and recombinant enzyme from Escherichia coli, amino acid sequence determination | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-isoleucine + tRNAIle | - |
Pseudomonas fluorescens | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? | |
ATP + L-isoleucine + tRNAIle | tRNAIle from Pseudomonas fluorescens and Escherichia coli | Pseudomonas fluorescens | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? | |
ATP + L-isoleucine + tRNAIle | - |
Pseudomonas fluorescens NCIB 10586 | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? | |
ATP + L-isoleucine + tRNAIle | tRNAIle from Pseudomonas fluorescens and Escherichia coli | Pseudomonas fluorescens NCIB 10586 | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 117000-117738, native isozyme IleRS-R2, SDS-PAGE and amino acid sequence determination | Pseudomonas fluorescens |
Synonyms | Comment | Organism |
---|---|---|
IleRS | - |
Pseudomonas fluorescens |
IRS | - |
Pseudomonas fluorescens |
Isoleucine translase | - |
Pseudomonas fluorescens |
Isoleucine--tRNA ligase | - |
Pseudomonas fluorescens |
Isoleucine-transfer RNA ligase | - |
Pseudomonas fluorescens |
Isoleucine-tRNA synthetase | - |
Pseudomonas fluorescens |
Isoleucyl-transfer ribonucleate synthetase | - |
Pseudomonas fluorescens |
Isoleucyl-transfer RNA synthetase | - |
Pseudomonas fluorescens |
Isoleucyl-tRNA synthetase | - |
Pseudomonas fluorescens |
Mupirocin resistance protein | - |
Pseudomonas fluorescens |
Synthetase, isoleucyl-transfer ribonucleate | - |
Pseudomonas fluorescens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
L-isoleucine | pH 7.5, recombinant isozyme IleRS-R1 | Pseudomonas fluorescens | |
2.64 | - |
L-isoleucine | pH 7.5, recombinant isozyme IleRS-R2 | Pseudomonas fluorescens | |
3 | 6 | L-isoleucine | pH 7.5, recombinant isozyme IleRS-R2 | Pseudomonas fluorescens | |
3.3 | - |
L-isoleucine | pH 7.5, native isozyme IleRS-R2 | Pseudomonas fluorescens | |
4.63 | - |
L-isoleucine | pH 7.5, native isozyme IleRS-R2 | Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Pseudomonas fluorescens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000105 | - |
pseudomonic acid | pH 7.5, recombinant isozyme IleRS-R1, versus L-isoleucine | Pseudomonas fluorescens | |
0.000015 | - |
pseudomonic acid | pH 7.5, recombinant isozyme IleRS-R1, versus ATP | Pseudomonas fluorescens |