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Literature summary for 6.1.1.5 extracted from
- Studies on isoleucyl-tRNA synthetase of an extreme thermophile, Thermus thermophilus HB8 (1975), Agric. Biol. Chem., 39, 1573-1580.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| K+ | - |
Thermus thermophilus |  |
| Na+ | - |
Thermus thermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0001 | - |
tRNAIle | - |
Thermus thermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Thermus thermophilus | |
| Mg2+ | optimal concentration is 10 mM | Thermus thermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 115000 | - |
gel filtration | Thermus thermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | - |
HB8 | - |
| Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
HB8 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Thermus thermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 73.8 | - |
- |
Thermus thermophilus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50% glycerol | Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-isoleucine + tRNAIle | - |
Thermus thermophilus | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? | |
| ATP + L-isoleucine + tRNAIle | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? | |
| additional information | Ile + ATP + enzyme/Ile-AMP-enzyme + diphosphate, isoleucine-dependent ATP-diphosphate exchange | Thermus thermophilus | ? | - |
? | |
| additional information | Ile + ATP + enzyme/Ile-AMP-enzyme + diphosphate, isoleucine-dependent ATP-diphosphate exchange | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 62 | - |
isoleucine-tRNA formation with E. coli tRNA | Thermus thermophilus |
| 75 | - |
isoleucine tRNA formation with Thermus thermophilus tRNA | Thermus thermophilus |
| 80 | - |
isoleucine dependent ATP-diphosphate exchange | Thermus thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 77 | - |
half-life is 15 min, inactivation is completely suppressed by addition of either E. coli or Thermus thermophilus tRNA | Thermus thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.7 | - |
- |
Thermus thermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.8 | about 30% of maximal activity at pH 6.0 and 8.8 | Thermus thermophilus |
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