Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination of residues 1-183, expression in Escherichia coli strain BL21(DE3) | Pyrococcus abyssi |
Crystallization (Comment) | Organism |
---|---|
purified ultrafiltrated recombinant residues 1-183, hanging drop vapour diffusion method, protein solution, containing 50 mM Tris-HCl, pH 7.5, and 50 mM NaCl, drops of varying volumes against 0.75 ml reservoir solution, at room temperature or at 4°C, preliminary X-ray diffraction for structure determination at 1.95 A resolution | Pyrococcus abyssi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-threonine + tRNAThr | Pyrococcus abyssi | - |
AMP + diphosphate + L-threonyl-tRNAThr | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus abyssi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant residues 1-183 from Escherichia coli strain BL21(DE3) by ion exchange chromatography, ammonium sulfate fractionation and gel filtration to homogeneity | Pyrococcus abyssi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-threonine + tRNAThr | - |
Pyrococcus abyssi | AMP + diphosphate + L-threonyl-tRNAThr | - |
r | |
ATP + L-threonine + tRNAThr | editing mechanism | Pyrococcus abyssi | AMP + diphosphate + L-threonyl-tRNAThr | - |
r |
Synonyms | Comment | Organism |
---|---|---|
Threonyl-tRNA synthetase | - |
Pyrococcus abyssi |
ThrRS | - |
Pyrococcus abyssi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Pyrococcus abyssi | |
ATP | - |
Pyrococcus abyssi |