Literature summary for 6.1.1.3 extracted from

Torres-Larios, A.; Dock-Bregeon, A.C.; Romby, P.; Rees, B.; Sankaranarayanan, R.; Caillet, J.; Springer, M.; Ehresmann, C.; Ehresmann, B.; Moras, D.
Structural basis of translational control by Escherichia coli threonyl tRNA synthetase (2002), Nat. Struct. Biol., 9, 343-347.

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme core DELTAN complexed with the essential operator domain mRNA, precipitation with 1.9 M ammonum sulfate in sodium cacodylate, pH 6.6, 4°C from solution containing 0.088 mM enzyme mutant DELTAN, 0.2 mM operator mRNA domain 2, 10 mM MgCl2, 10 mM substrate analogue 5'-O-(N-(L-threonyl)-sulfamoyl)adenosine, crystals are soaked in 25% v/v glycerol, X-ray diffraction structure determination at 3.6 A resolution and structure analysis	Escherichia coli

Crystallization (Comment)

Organism

enzyme core DELTAN complexed with the essential operator domain mRNA, precipitation with 1.9 M ammonum sulfate in sodium cacodylate, pH 6.6, 4°C from solution containing 0.088 mM enzyme mutant DELTAN, 0.2 mM operator mRNA domain 2, 10 mM MgCl2, 10 mM substrate analogue 5'-O-(N-(L-threonyl)-sulfamoyl)adenosine, crystals are soaked in 25% v/v glycerol, X-ray diffraction structure determination at 3.6 A resolution and structure analysis

Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of a truncated enzyme: core domain DELTAN comprising residues 242-642	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
operator mRNA domain 2	-	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00003	-	tRNAThr	wild-type enzyme	Escherichia coli
0.00043	-	tRNAThr	truncated enzyme core DELTAN	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-threonine + tRNAThr	Escherichia coli	regulatory mechanism	AMP + diphosphate + L-threonyl-tRNAThr	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0A8M3	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ATP + L-threonine + tRNAThr	-	Escherichia coli	AMP + diphosphate + L-threonyl-tRNAThr	-	?
ATP + L-threonine + tRNAThr	regulatory mechanism	Escherichia coli	AMP + diphosphate + L-threonyl-tRNAThr	-	?
additional information	the enzyme represses the translation of its own mRNA by binding to an operator located upstream of the initiation codon thereby using the recognition mode of the tRNA anticodon loop to initiate binding	Escherichia coli	?	-	?

Synonyms

Synonyms	Comment	Organism
Synthetase, threonyl-transfer ribonucleate	-	Escherichia coli
Threonine translase	-	Escherichia coli
Threonine--tRNA ligase	-	Escherichia coli
Threonine-transfer ribonucleate synthetase	-	Escherichia coli
Threonyl-ribonucleic synthetase	-	Escherichia coli
Threonyl-transfer ribonucleate synthetase	-	Escherichia coli
Threonyl-transfer ribonucleic acid synthetase	-	Escherichia coli
Threonyl-transfer RNA synthetase	-	Escherichia coli
Threonyl-tRNA synthetase	-	Escherichia coli
ThrRS	-	Escherichia coli
TRS	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Escherichia coli