Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
enzyme complexed with ATP and threonine and complexed with substrate analogue 5'-O-(N-(L-threonyl)-sulfamoyl)adenosine, hanging drop method, 15 mg/ml protein in 20 mM HEPES, pH 7.2, 2 mM MgCl2, 100 mM KCl, in a 1:1:1 mixture with ligand solution containing 10 mM MgCl2, 10 mM ATP or 1 mM Thr-AMS, and well solution containing 50 mM Tris-HCl, pH 7.5-8.5, PEG 8000 12-14% w/v, and ammonium acetate or potassium chloride in the range 0.2-0.4 M, 2-3 days at 4°C, X-ray diffraction structure determination at resolution 2.8-3.2 A, structure analysis, modeling | Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-threonine + tRNAThr | Staphylococcus aureus | - |
AMP + diphosphate + L-threonyl-tRNAThr | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | Q8NW68 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli | Staphylococcus aureus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-threonine + tRNAThr = AMP + diphosphate + L-threonyl-tRNAThr | substrate binding and mechanism | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-threonine + tRNAThr | - |
Staphylococcus aureus | AMP + diphosphate + L-threonyl-tRNAThr | - |
? | |
ATP + L-threonine + tRNAThr | substrate binding induces conformational changes | Staphylococcus aureus | AMP + diphosphate + L-threonyl-tRNAThr | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Synthetase, threonyl-transfer ribonucleate | - |
Staphylococcus aureus |
Threonine translase | - |
Staphylococcus aureus |
Threonine--tRNA ligase | - |
Staphylococcus aureus |
Threonine-transfer ribonucleate synthetase | - |
Staphylococcus aureus |
Threonyl-ribonucleic synthetase | - |
Staphylococcus aureus |
Threonyl-transfer ribonucleate synthetase | - |
Staphylococcus aureus |
Threonyl-transfer ribonucleic acid synthetase | - |
Staphylococcus aureus |
Threonyl-transfer RNA synthetase | - |
Staphylococcus aureus |
Threonyl-tRNA synthetase | - |
Staphylococcus aureus |
ThrRS | - |
Staphylococcus aureus |
TRS | - |
Staphylococcus aureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
assay at | Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Staphylococcus aureus |