Crystallization (Comment) | Organism |
---|---|
lamdaN-threonine-tRNA ligase complexed with Ser-AMS, X-ray diffraction structure determination at 1.65 A resolution and analysis | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D180A | charging of tRNAThr with serine, mutant is no longer able to rapidly deacetylate Ser-tRNAThr | Escherichia coli |
H73A/H77A | charging of tRNAThr with serine, mutant is no longer able to deacetylate Ser-tRNAThr | Escherichia coli |
additional information | truncated lamdaN-enzyme mutant, lacking the N-terminal domains N1 and N2, produces Ser-tRNAThr, reduced activity and altered substrate recognition compared to the wild-type which does nearly not incorporate serine | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | used to discriminate against the isosteric valine at the activation step | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-threonine + tRNAThr | Escherichia coli | - |
AMP + diphosphate + L-threonyl-tRNAThr | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
class II enzyme | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-threonine + tRNAThr = AMP + diphosphate + L-threonyl-tRNAThr | functional mechanism and substrate recognition, editing model, 2 separate active sites for substrate binding, binding of tRNA is more specific than the binding of the amino acid | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-serine + tRNAThr | very low activity with the wild-type enzyme | Escherichia coli | AMP + diphosphate + L-seryl-tRNAThr | - |
? | |
ATP + L-threonine + tRNAThr | - |
Escherichia coli | AMP + diphosphate + L-threonyl-tRNAThr | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Synthetase, threonyl-transfer ribonucleate | - |
Escherichia coli |
Threonine translase | - |
Escherichia coli |
Threonine--tRNA ligase | - |
Escherichia coli |
Threonine-transfer ribonucleate synthetase | - |
Escherichia coli |
Threonyl-ribonucleic synthetase | - |
Escherichia coli |
Threonyl-transfer ribonucleate synthetase | - |
Escherichia coli |
Threonyl-transfer ribonucleic acid synthetase | - |
Escherichia coli |
Threonyl-transfer RNA synthetase | - |
Escherichia coli |
Threonyl-tRNA synthetase | - |
Escherichia coli |
ThrRS | - |
Escherichia coli |
TRS | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |