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Literature summary for 6.1.1.3 extracted from
- Induced fit and kinetic mechanism of adenylation catalyzed by Escherichia coli threonyl-tRNA synthetase (2003), Biochemistry, 42, 15102-15113.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in strain IBPC6881 as His-tagged enzyme | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| W434Y | reduced activity, Trp434 is involved in conformational changes during substrate binding | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics and kinetic mechanism | Escherichia coli | |
| 0.201 | - |
L-threonine | wild-type enzyme, pH 7.2, 37°C | Escherichia coli |  |
| 0.267 | - |
ATP | wild-type enzyme, pH 7.2, 37°C | Escherichia coli | |
| 0.387 | - |
ATP | mutant enzyme W434Y, pH 7.2, 37°C | Escherichia coli | |
| 0.897 | - |
L-threonine | mutant enzyme W434Y, pH 7.2, 37°C | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-threonine + tRNAThr | Escherichia coli | - |
AMP + diphosphate + L-threonyl-tRNAThr | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from strain IBPC6881, to homogeneity | Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + L-threonine + tRNAThr = AMP + diphosphate + L-threonyl-tRNAThr | induced fit, Trp434 is involved in conformational changes during substrate binding | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-threonine + tRNAThr | - |
Escherichia coli | AMP + diphosphate + L-threonyl-tRNAThr | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Synthetase, threonyl-transfer ribonucleate | - |
Escherichia coli |
| Threonine translase | - |
Escherichia coli |
| Threonine--tRNA ligase | - |
Escherichia coli |
| Threonine-transfer ribonucleate synthetase | - |
Escherichia coli |
| Threonyl-ribonucleic synthetase | - |
Escherichia coli |
| Threonyl-transfer ribonucleate synthetase | - |
Escherichia coli |
| Threonyl-transfer ribonucleic acid synthetase | - |
Escherichia coli |
| Threonyl-transfer RNA synthetase | - |
Escherichia coli |
| Threonyl-tRNA synthetase | - |
Escherichia coli |
| ThrRS | - |
Escherichia coli |
| TRS | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 30 | - |
L-threonine | mutant enzyme W434Y, pH 7.2, 37°C | Escherichia coli | |
| 42 | - |
ATP | mutant enzyme W434Y, pH 7.2, 37°C | Escherichia coli | |
| 90 | - |
ATP | wild-type enzyme, pH 7.2, 37°C | Escherichia coli | |
| 90 | - |
L-threonine | wild-type enzyme, pH 7.2, 37°C | Escherichia coli | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Escherichia coli | |
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