Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.3 extracted from

  • Dignam, J.D.; Rhodes, D.G.; Deutscher, M.P.
    Purification and structural characterization of rat liver threonyl transfer ribonucleic acid synthetase (1980), Biochemistry, 19, 4978-4984.
    View publication on PubMed
Search for more literature for 6.1.1.3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 tRNAThr
-
 Rattus norvegicus
0.4
-
 2-Chloroadenosine 5'-triphosphate ATP Rattus norvegicus
0.5
-
 Formycin 5'-triphosphate threonine Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
 2 * 85000, SDS-PAGE Rattus norvegicus
154000
-
 sedimentation equilibrium measurement Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.7
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
-80°C, about 30% loss of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-Chloroadenosine 5'-triphosphate + L-threonine + tRNAThr
-
 Rattus norvegicus ?
-
 ?
ATP + L-threonine + tRNAThr
-
 Rattus norvegicus AMP + diphosphate + L-threonyl-tRNAThr
-
 ?
Formycin 5'-triphosphate + L-threonine + tRNAThr
-
 Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 85000, SDS-PAGE Rattus norvegicus