Literature summary for 6.1.1.21 extracted from

Su, D.; Lieberman, A.; Lang, B.F.; Simonovic, M.; Soell, D.; Ling, J.
An unusual tRNAThr derived from tRNAHis reassigns in yeast mitochondria the CUN codons to threonine (2011), Nucleic Acids Res., 39, 4866-4874.

Search for more literature for 6.1.1.21

Cloned(Commentary)

Cloned (Comment)	Organism
HTS1, phylogenetic analysis, expression in Escherichia coli strain BL21(DE3)	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00043	-	tRNAHis	pH 7.2, 37°C, wild-type substrate	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Saccharomyces cerevisiae	5739	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-histidine + tRNAHis	Saccharomyces cerevisiae	-	AMP + diphosphate + L-histidyl-tRNAHis	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-histidine + tRNAHis	-	Saccharomyces cerevisiae	AMP + diphosphate + L-histidyl-tRNAHis	-	?
ATP + L-histidine + tRNAHis	active with wild-type and mutant C73A and A37 insertion tRNAHis	Saccharomyces cerevisiae	AMP + diphosphate + L-histidyl-tRNAHis	-	?
additional information	addition of a first nucleotide to tRNAThr1 allows efficient histidylation by histidyl-tRNA synthetase. Loss of the first nucleotide of wild-type tRNAHis converts it to a substrate for threonyl-tRNA sythetase, EC 6.1.1.3	Saccharomyces cerevisiae	?	-	?

Synonyms

Synonyms	Comment	Organism
Histidyl-tRNA synthetase	-	Saccharomyces cerevisiae
HTS1	-	Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.417	-	tRNAHis	pH 7.2, 37°C, wild-type substrate	Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	assay at	Saccharomyces cerevisiae

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Saccharomyces cerevisiae

General Information

General Information	Comment	Organism
evolution	phylogenetic analysis reveals that tRNAThr1 originated from tRNAHis	Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1000	-	tRNAHis	pH 7.2, 37°C, wild-type substrate	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)