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Literature summary for 6.1.1.21 extracted from

  • Merritt, E.A.; Arakaki, T.L.; Gillespie, J.R.; Larson, E.T.; Kelley, A.; Mueller, N.; Napuli, A.J.; Kim, J.; Zhang, L.; Verlinde, C.L.; Fan, E.; Zucker, F.; Buckner, F.S.; van Voorhis, W.C.; Hol, W.G.
    Crystal structures of trypanosomal histidyl-tRNA synthetase illuminate differences between eukaryotic and prokaryotic homologs (2010), J. Mol. Biol., 397, 481-494.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.21

Cloned(Commentary)

Cloned (Comment) Organism
the full-length and a truncated variant is cloned into the Escherichia coli expression vector AVA0421 derived from vector pET14b Trypanosoma brucei
the full-length and a truncated variant is cloned into the Escherichia coli expression vector AVA0421 derived from vector pET14b Trypanosoma cruzi

Crystallization (Commentary)

Crystallization (Comment) Organism
the structure of histidyl-tRNA synthetase in complex with L-histidine is determined to a resolution of 1.8 A , in complex with histidyl-adenosinemonophosphate to a resolution of 3.05 A Trypanosoma cruzi
the structure of histidyl-tRNA synthetase is determined to a resolution of 2.7 A Trypanosoma brucei

Inhibitors

Inhibitors Comment Organism Structure
RNAi
-
 Trypanosoma brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-histidine + tRNAHis Trypanosoma brucei
-
 AMP + diphosphate + L-histidyl-tRNAHis
-
 ?
ATP + L-histidine + tRNAHis Trypanosoma cruzi
-
 AMP + diphosphate + L-histidyl-tRNAHis
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei Q4DA54 residues 44-477
-
Trypanosoma cruzi Q4DA54 residues 45-478
-

Purification (Commentary)

Purification (Comment) Organism
by a Ni-NTA affinity column followed by a XK26/60 Superdex 75 column Trypanosoma brucei
by a Ni-NTA affinity column followed by a XK26/60 Superdex 75 column Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-histidine + tRNAHis
-
 Trypanosoma brucei AMP + diphosphate + L-histidyl-tRNAHis
-
 ?
ATP + L-histidine + tRNAHis
-
 Trypanosoma cruzi AMP + diphosphate + L-histidyl-tRNAHis
-
 ?

Synonyms

Synonyms Comment Organism
HisRS
-
 Trypanosoma brucei
HisRS
-
 Trypanosoma cruzi
Histidyl-tRNA synthetase
-
 Trypanosoma brucei
Histidyl-tRNA synthetase
-
 Trypanosoma cruzi

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Trypanosoma brucei
ATP
-
 Trypanosoma cruzi