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Literature summary for 6.1.1.21 extracted from

  • Guth, E.; Farris, M.; Bovee, M.; Francklyn, C.S.
    Asymmetric amino acid activation by class II histidyl-tRNA synthetase from Escherichia coli (2009), J. Biol. Chem., 284, 20753-20762.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.21

Application

Application Comment Organism
analysis alternating catalysis requires a mechanism for coupling events between active sites, presumably through conformational changes propagated between these active sites, a version of HisRS is developed that features the site-specific incorporation of extrinsic environmentally sensitive fluorescent probes, allowing the adenylation reaction to be followed by stopped-flow fluorometry Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
the vectors pWY30 anf pQE30 are used Escherichia coli

Protein Variants

Protein Variants Comment Organism
C88V/C196S/C241L denoted as clHisRS, can not be labeled with maleimide probes Escherichia coli
L276C clHisRS an additional cysteine is introduced at a solvent-accessible position proximal to substrate binding sites Escherichia coli
L402C clHisRS an additional cysteine is introduced at a solvent-accessible position proximal to substrate binding sites Escherichia coli
MDCC-HisRS fluorescently labeled version of HisRS, conjugated with 7-diethylamino-3-((((2-maleimidyl)ethyl)amino)carbonyl)-coumarin, MDCC, to a cysteine introduced at residue 212, located in the insertion domain Escherichia coli
N212C clHisRS an additional cysteine is introduced at a solvent-accessible position proximal to substrate binding sites Escherichia coli
N368C clHisRS an additional cysteine is introduced at a solvent-accessible position proximal to substrate binding sites Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-histidine + tRNAHis Escherichia coli
-
 AMP + diphosphate + L-histidyl-tRNAHis
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P60906
-
-

Purification (Commentary)

Purification (Comment) Organism
employing Q-Sepharose and hydroxyapatite chromatography, by utilizing the His-tag Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-histidine + tRNAHis
-
 Escherichia coli AMP + diphosphate + L-histidyl-tRNAHis
-
 ?

Synonyms

Synonyms Comment Organism
Histidyl-tRNA synthetase
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli