Protein Variants | Comment | Organism |
---|---|---|
additional information | mutational study of the 1:73 base pair recognition by yeats HisRS using two chemically synthesized 24-nucleotide RNA microhelices, overview | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00014 | - |
tRNAHis | pH 8.0, 37°C, aminoacylation | Saccharomyces cerevisiae | |
0.0038 | - |
RNA microhelix | pH 8.0, 37°C, aminoacylation | Escherichia coli | |
0.009 | - |
L-histidine | pH 8.0, 37°C, diphosphate exchange | Saccharomyces cerevisiae | |
0.03 | - |
L-histidine | pH 8.0, 37°C, diphosphate exchange | Escherichia coli | |
0.034 | - |
tRNAHis | pH 8.0, 37°C, aminoacylation | Escherichia coli | |
0.124 | - |
RNA microhelix | pH 8.0, 37°C, aminoacylation | Saccharomyces cerevisiae | |
0.5 | - |
ATP | pH 8.0, 37°C, diphosphate exchange | Saccharomyces cerevisiae | |
0.89 | - |
ATP | pH 8.0, 37°C, diphosphate exchange | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli | |
Mg2+ | - |
Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-histidine + tRNAHis | Escherichia coli | - |
AMP + diphosphate + L-histidinyl-tRNAHis | - |
? | |
ATP + L-histidine + tRNAHis | Saccharomyces cerevisiae | - |
AMP + diphosphate + L-histidinyl-tRNAHis | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
additional information | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-histidine + RNA microhelix | - |
Escherichia coli | ? | - |
? | |
ATP + L-histidine + RNA microhelix | - |
Saccharomyces cerevisiae | ? | - |
? | |
ATP + L-histidine + tRNAHis | - |
Escherichia coli | AMP + diphosphate + L-histidinyl-tRNAHis | - |
? | |
ATP + L-histidine + tRNAHis | - |
Saccharomyces cerevisiae | AMP + diphosphate + L-histidinyl-tRNAHis | - |
? | |
ATP + L-histidine + tRNAHis | all histidine tRNA molecules have an extra nucleotide, G-1, at the 59 end of the acceptor stem, G-1:C73 base pair binding analysis of the yeast enzyme | Saccharomyces cerevisiae | AMP + diphosphate + L-histidinyl-tRNAHis | - |
? | |
ATP + L-histidine + tRNAHis | all histidine tRNA molecules have an extra nucleotide, G-1, at the 59 end of the acceptor stem, importance of the G-1:C73 base pair to tRNAHisc, identity, specifically the 59-monophosphate of G-1 and the major groove amine of C73 are recognized by the enzyme of Escherichia coli stabilizing the reaction transition state | Escherichia coli | AMP + diphosphate + L-histidinyl-tRNAHis | - |
? | |
additional information | binding of a chemically synthesized 24-nucleotide RNA microhelix, which recapitulates the acceptor stem of Escherichia coli tRNAHis, functional group analysis, overview | Escherichia coli | ? | - |
? | |
additional information | binding of a chemically synthesized 24-nucleotide RNA microhelix, which recapitulates the acceptor stem of Saccharomyces cervisiae tRNAHis, functional group analysis, overview | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HisRS | - |
Escherichia coli |
HisRS | - |
Saccharomyces cerevisiae |
histidyl-transfer RNA synthetase | - |
Escherichia coli |
histidyl-transfer RNA synthetase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
aminoacylation assay at | Escherichia coli |
30 | - |
aminoacylation assay at | Saccharomyces cerevisiae |
37 | - |
diphosphate exchange assay at | Escherichia coli |
37 | - |
diphosphate exchange assay at | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
RNA microhelix | pH 8.0, 37°C, aminoacylation | Saccharomyces cerevisiae | |
0.024 | - |
RNA microhelix | pH 8.0, 37°C, aminoacylation | Escherichia coli | |
0.42 | - |
tRNAHis | pH 8.0, 37°C, aminoacylation | Saccharomyces cerevisiae | |
1.71 | - |
tRNAHis | pH 8.0, 37°C, aminoacylation | Escherichia coli | |
40 | - |
ATP | pH 8.0, 37°C, diphosphate exchange | Saccharomyces cerevisiae | |
40 | - |
L-histidine | pH 8.0, 37°C, diphosphate exchange | Saccharomyces cerevisiae | |
130 | - |
ATP | pH 8.0, 37°C, diphosphate exchange | Escherichia coli | |
130 | - |
L-histidine | pH 8.0, 37°C, diphosphate exchange | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
aminoacylation assay at | Escherichia coli |
7.5 | - |
aminoacylation assay at | Saccharomyces cerevisiae |
8 | - |
diphosphate exchange assay at | Escherichia coli |
8 | - |
diphosphate exchange assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli | |
ATP | - |
Saccharomyces cerevisiae |