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Literature summary for 6.1.1.21 extracted from

  • Kalousek, F.; Konigsberg, W.H.
    Purification and characterization of histidyl transfer ribonucleic acid synthetase of Escherichia coli (1974), Biochemistry, 13, 999-1006.
    View publication on PubMed

General Stability

General Stability Organism
2-mercaptoethanol stabilizes the enzyme Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
1,2,4-Triazole-3-alanine
-
Escherichia coli
D-histidine
-
Escherichia coli
diphosphate weak Escherichia coli
L-histidinol
-
Escherichia coli
Mg2+ inhibition at 15 mM, optimal activation at 5-10 mM Escherichia coli
N-Acetylhistidine
-
Escherichia coli
NEM
-
Escherichia coli
p-chloromercuribenzoate L-His, ATP, and Mg2+ protect Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimal concentration: 5-10 mM, inhibition at 15 mM Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42500
-
2 * 42500, SDS-PAGE Escherichia coli
85000
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
K12
-

Reaction

Reaction Comment Organism Reaction ID
ATP + L-histidine + tRNAHis = AMP + diphosphate + L-histidyl-tRNAHis two molecules of ATP and histidine are bound per molecule of enzyme Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
121.8
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol, 5 mM 2-mercaproethanol, stable for more than 4 months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-histidine + tRNAHis
-
Escherichia coli AMP + diphosphate + L-histidyl-tRNAHis
-
?

Subunits

Subunits Comment Organism
dimer 2 * 42500, SDS-PAGE Escherichia coli
More at temperatures above 25 C, the enzyme tends to aggregate giving forms with a MW 90000 and 175000 Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
in presence of 2-mercaptoethanol stable for 5 h, 40% loss of activity after 12 h. In absence of 2-mercaptoethanol total loss of activity in a few min Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
histidyl-tRNA formation Escherichia coli
8 9 histidine-dependent ATP-diphosphate exchange Escherichia coli