Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | activates | Escherichia coli |
Application | Comment | Organism |
---|---|---|
biotechnology | misacylation of suppressor tRNAPhe CUA by the PheRS mutant A294G with 4-iodo-L-phenylalanine might be useful in application in protein engineering since an aryl iodide tag on proteins can be used for site-specific functionalization of proteins, used for cell-free protein synthesis as a stoichiometric reagent, overview | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
A294G | thermosensitive active site mutant, the suppressor tRNAPhe CUA is misacylated with 4-iodo-L-phenylalanine by the mutant at a high magnesium-ion concentration of 70 mM | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-phenylalanine + tRNAPhe | Escherichia coli | - |
AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
ATP + L-phenylalanine + tRNAPhe | Escherichia coli NP37 | - |
AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
carrying the thermosensitive point mutation A294G in the active site | - |
Escherichia coli NP37 | - |
carrying the thermosensitive point mutation A294G in the active site | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-iodo-L-phenylalanine + amber tRNAPheCUA | suppressor tRNAPhe CUA is misacylated with 4-iodo-L-phenylalanine by the mutant at a high magnesium-ion concentration by PheRS mutant A294G | Escherichia coli | AMP + diphosphate + 4-iodo-L-phenylalanyl-amber tRNAPheCUA | - |
? | |
ATP + 4-iodo-L-phenylalanine + amber tRNAPheCUA | suppressor tRNAPhe CUA is misacylated with 4-iodo-L-phenylalanine by the mutant at a high magnesium-ion concentration by PheRS mutant A294G | Escherichia coli NP37 | AMP + diphosphate + 4-iodo-L-phenylalanyl-amber tRNAPheCUA | - |
? | |
ATP + L-phenylalanine + tRNAPhe | - |
Escherichia coli | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
ATP + L-phenylalanine + tRNAPhe | - |
Escherichia coli NP37 | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Phenylalanyl-tRNA synthetase | - |
Escherichia coli |
PheRS | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.1 | assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |