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Literature summary for 6.1.1.20 extracted from

  • Roy, H.; Ling, J.; Alfonzo, J.; Ibba, M.
    Loss of editing activity during the evolution of mitochondrial phenylalanyl-tRNA synthetase (2005), J. Biol. Chem., 280, 38186-18192.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0042
-
L-Phe pH 7.2, 37°C, mitochondrial enzyme Saccharomyces cerevisiae
0.03
-
L-Phe pH 7.2, 37°C, cytosolic enzyme Saccharomyces cerevisiae
0.86
-
L-Tyr pH 7.2, 37°C, cytosolic enzyme Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Saccharomyces cerevisiae 5829
-
mitochondrion
-
Saccharomyces cerevisiae 5739
-

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Phe + tRNAPhe
-
Saccharomyces cerevisiae AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
?
ATP + L-Tyr + tRNAPhe cytosolic PheRS contains an editing site, which upon disruption abolishes both cis and trans editing of Tyr-tRNAPhe. Wild-type mitochondrial PheRS lacks cis and trans editing and can synthesisze Tyr-tRNAPhe Saccharomyces cerevisiae AMP + diphosphate + L-tyrosyl-tRNAPhe
-
?

Synonyms

Synonyms Comment Organism
PheRS
-
Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
150
-
L-Phe pH 7.2, 37°C, mitochondrial enzyme Saccharomyces cerevisiae
160
-
L-Tyr pH 7.2, 37°C, cytosolic enzyme Saccharomyces cerevisiae
240
-
L-Phe pH 7.2, 37°C, cytosolic enzyme Saccharomyces cerevisiae