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Literature summary for 6.1.1.20 extracted from

  • Roy, H.; Ling, J.; Irnov, M.; Ibba, M.
    Post-transfer editing in vitro and in vivo by the beta subunit of phenylalanyl-tRNA synthetase (2004), EMBO J., 23, 4639-4648.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.20

Protein Variants

Protein Variants Comment Organism
A249G/A356W mutant enzyme with A294G mutation in alpha-subunit and A356W mutation in beta-subunit, kcat/Km is 6.6% of wild-type value Escherichia coli
A249G/E334A mutant enzyme with A294G mutation in alpha-subunit and E334A mutation in beta-subunit, kcat/Km is 60% of wild-type value Escherichia coli
A249G/H265A mutant enzyme with A294G mutation in alpha-subunit and H265A mutation in beta-subunit, kcat/Km is 52% of wild-type value Escherichia coli
A249G/H265L mutant enzyme with A294G mutation in alpha-subunit and H265L mutation in beta-subunit, kcat/Km is 63% of wild-type value Escherichia coli
A249G/T354W mutant enzyme with A294G mutation in alpha-subunit and T354W mutation in beta-subunit, kcat/Km is 26% of wild-type value Escherichia coli
A294G mutant enzyme with A294G mutation in alpha-subunit, kcat/Km is 58% of wild-type value Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0103
-
 L-Phe wild-type enzyme Escherichia coli
0.0212
-
 L-Phe mutant enzyme with A294G mutation in alpha-subunit Escherichia coli
0.0225
-
 L-Phe mutant enzyme with A294G mutation in alpha-subunit and T354W mutation in beta-subunit Escherichia coli
0.0261
-
 L-Phe mutant enzyme with A294G mutation in alpha-subunit and E334A mutation in beta-subunit Escherichia coli
0.0282
-
 L-Phe mutant enzyme with A294G mutation in alpha-subunit and A356W mutation in beta-subunit Escherichia coli
0.0312
-
 L-Phe mutant enzyme with A294G mutation in alpha-subunit and H265L mutation in beta-subunit Escherichia coli
0.0331
-
 L-Phe mutant enzyme with A294G mutation in alpha-subunit and H265A mutation in beta-subunit Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli phenylalanyl-tRNA synthetase misactivates tyrosine and subsequently corrects such errors through hydrolysis of tyrosyl-adenylate and Tyr-tRNAPhe. Editing by phenylalanyl-tRNA synthetase is essential for faithful translation of the genetic code ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Phe + tRNAPhe
-
 Escherichia coli AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
additional information phenylalanyl-tRNA synthetase misactivates tyrosine and subsequently corrects such errors through hydrolysis of tyrosyl-adenylate and Tyr-tRNAPhe. Editing by phenylalanyl-tRNA synthetase is essential for faithful translation of the genetic code Escherichia coli ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.975
-
 L-Phe mutant enzyme with A294G mutation in alpha-subunit and T354W mutation in beta-subunit Escherichia coli
1.72
-
 L-Phe wild-type enzyme Escherichia coli
2.04
-
 L-Phe mutant enzyme with A294G mutation in alpha-subunit Escherichia coli
2.645
-
 L-Phe mutant enzyme with A294G mutation in alpha-subunit and E334A mutation in beta-subunit Escherichia coli
2.88
-
 L-Phe mutant enzyme with A294G mutation in alpha-subunit and H265A mutation in beta-subunit Escherichia coli
3 6 L-Phe mutant enzyme with A294G mutation in alpha-subunit and E334A mutation in beta-subunit Escherichia coli
3.12
-
 L-Phe mutant enzyme with A294G mutation in alpha-subunit and A356W mutation in beta-subunit Escherichia coli
3.3
-
 L-Phe mutant enzyme with A294G mutation in alpha-subunit and H265L mutation in beta-subunit Escherichia coli
6.08
-
 L-Phe mutant enzyme with A294G mutation in alpha-subunit and T354W mutation in beta-subunit Escherichia coli