Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination, individual cloning and coexpression of His-tagged alpha- and beta-subunits in Escherichia coli resulting in 3 different types of (alphabeta)2 heterodimers with the His-tag at the N-terminus, N-terminally His-tagged beta-subunit renders the enzyme inactive, overexpression of nontagged alpha- and beta-subunits in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the N-terminal His-tag does not influence the kinetic parameters of tRNAPhe aminoacylation, cleavage of the His-tag by thrombin leads to nonspecific splitting of the enzyme that occurs in parallel to the main reaction | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0001 | - |
tRNAPhe | recombinant heterodimer, pH 8.0, 25°C | Homo sapiens | |
0.00011 | - |
tRNAPhe | recombinant His-tagged heterodimer, pH 8.0, 25°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
2 * 57000, alpha-subunit, + 2 * 66000, beta-subunit, (alphabeta)2, SDS-PAGE | Homo sapiens |
66000 | - |
2 * 57000, alpha-subunit, + 2 * 66000, beta-subunit, (alphabeta)2, SDS-PAGE | Homo sapiens |
297000 | - |
recombinant enzyme, dynamic light scattering at 20° | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-phenylalanine + tRNAPhe | Homo sapiens | - |
AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged heterodimers and nontagged subunits from Escherichia coli, over 200fold | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-phenylalanine + tRNAPhe | - |
Homo sapiens | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
ATP + L-phenylalanine + tRNAPhe | the N-terminal coiled-coil structure of the alpha-subunit is involved in the binding of cognate tRNAPhe | Homo sapiens | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 57000, alpha-subunit, + 2 * 66000, beta-subunit, (alphabeta)2, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
CML33 | - |
Homo sapiens |
FRS | - |
Homo sapiens |
HSPC173 | - |
Homo sapiens |
L-Phenylalanyl-tRNA synthetase | - |
Homo sapiens |
Phenylalanine translase | - |
Homo sapiens |
Phenylalanine--tRNA ligase | - |
Homo sapiens |
Phenylalanine-tRNA synthetase | - |
Homo sapiens |
Phenylalanyl transfer ribonucleic acid synthetase | - |
Homo sapiens |
Phenylalanyl-transfer ribonucleate synthetase | - |
Homo sapiens |
Phenylalanyl-transfer RNA ligase | - |
Homo sapiens |
Phenylalanyl-transfer RNA synthetase | - |
Homo sapiens |
Phenylalanyl-tRNA ligase | - |
Homo sapiens |
Phenylalanyl-tRNA synthetase | - |
Homo sapiens |
PheRS | - |
Homo sapiens |
Synthetase, phenylalanyl-transfer ribonucleate | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |