Activating Compound | Comment | Organism | Structure |
---|---|---|---|
elongation factor 1alpha | interaction enhances the activity | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00048 | - |
L-phenylalanine | pH 7.8, 60°C | Saccharolobus solfataricus | |
0.0015 | - |
L-phenylalanine | pH 7.8, 70°C | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
2 * 56000, alpha-subunit, + 2 * 64000, beta-subunit, (alphabeta)2, SDS-PAGE | Saccharolobus solfataricus |
64000 | - |
2 * 56000, alpha-subunit, + 2 * 64000, beta-subunit, (alphabeta)2, SDS-PAGE | Saccharolobus solfataricus |
240000 | - |
gel filtration | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-phenylalanine + tRNAPhe | Saccharolobus solfataricus | enzyme is essemtial for poly(Phe) synthesis | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
60fold | Saccharolobus solfataricus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-phenylalanine + tRNAPhe = AMP + diphosphate + L-phenylalanyl-tRNAPhe | tRNA channeling mechanism | Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-phenylalanine + tRNAPhe | activity is restricted to endogenous tRNA, highly specific for L-phenylalanine | Saccharolobus solfataricus | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
ATP + L-phenylalanine + tRNAPhe | enzyme is essemtial for poly(Phe) synthesis | Saccharolobus solfataricus | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
GTP + L-phenylalanine + tRNAPhe | 40fold lower activity compared to ATP | Saccharolobus solfataricus | GMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 56000, alpha-subunit, + 2 * 64000, beta-subunit, (alphabeta)2, SDS-PAGE | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
CML33 | - |
Saccharolobus solfataricus |
FRS | - |
Saccharolobus solfataricus |
HSPC173 | - |
Saccharolobus solfataricus |
L-Phenylalanyl-tRNA synthetase | - |
Saccharolobus solfataricus |
Phenylalanine translase | - |
Saccharolobus solfataricus |
Phenylalanine--tRNA ligase | - |
Saccharolobus solfataricus |
Phenylalanine-tRNA synthetase | - |
Saccharolobus solfataricus |
Phenylalanyl transfer ribonucleic acid synthetase | - |
Saccharolobus solfataricus |
Phenylalanyl-transfer ribonucleate synthetase | - |
Saccharolobus solfataricus |
Phenylalanyl-transfer RNA ligase | - |
Saccharolobus solfataricus |
Phenylalanyl-transfer RNA synthetase | - |
Saccharolobus solfataricus |
Phenylalanyl-tRNA ligase | - |
Saccharolobus solfataricus |
Phenylalanyl-tRNA synthetase | - |
Saccharolobus solfataricus |
PheRS | - |
Saccharolobus solfataricus |
Synthetase, phenylalanyl-transfer ribonucleate | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 92 | - |
Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
88 | - |
10 min, 50% inactivation | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.055 | - |
L-phenylalanine | pH 7.8, 60°C | Saccharolobus solfataricus | |
0.115 | - |
L-phenylalanine | pH 7.8, 70°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
broad maximum | Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | not dependent on | Saccharolobus solfataricus | |
GTP | 40fold lower activity than with ATP | Saccharolobus solfataricus |