Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the rate of overcharging of the tRNAPhe is dependenton the buffer system used | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Thermus thermophilus | |
diphosphate | - |
Thermus thermophilus | |
additional information | the rate of overcharging of the tRNAPhe is dependenton the buffer system used | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-phenylalanine + tRNAPhe | Thermus thermophilus | - |
AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
ir | |
ATP + L-phenylalanine + tRNAPhe | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.21 | - |
purified enzyme, 37°C | Thermus thermophilus |
0.31 | - |
purified recombinant enzyme expressed in Escherichia coli, 37°C | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2 L-phenylalanine + tRNAPhe | mechanism, formation of bisphenylalanyl-tRNAPhe with tRNA substrates from Thermus thermophilus, isoacceptor I, and from Escherichia coli, yeast and human, the second phenylalanyl residue is attached to tRNA approximately 50 times more slowly than the first one, the presence of modified nucleotides is not necessary for tRNAPhe overcharging | Thermus thermophilus | AMP + diphosphate + bis-L-phenylalanyl-tRNAPhe | overcharged product cannot be isolated from living cells | ir | |
ATP + 2 L-phenylalanine + tRNAPhe | mechanism, formation of bisphenylalanyl-tRNAPhe with tRNA substrates from Thermus thermophilus, isoacceptor I, and from Escherichia coli, yeast and human, the second phenylalanyl residue is attached to tRNA approximately 50 times more slowly than the first one, the presence of modified nucleotides is not necessary for tRNAPhe overcharging | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + bis-L-phenylalanyl-tRNAPhe | overcharged product cannot be isolated from living cells | ir | |
ATP + L-phenylalanine + tRNAPhe | - |
Thermus thermophilus | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
ir | |
ATP + L-phenylalanine + tRNAPhe | tRNA substrates from Thermus thermophilus, isoacceptor I, and from Escherichia coli, yeast and human | Thermus thermophilus | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
ir | |
ATP + L-phenylalanine + tRNAPhe | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
ir | |
ATP + L-phenylalanine + tRNAPhe | tRNA substrates from Thermus thermophilus, isoacceptor I, and from Escherichia coli, yeast and human | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
CML33 | - |
Thermus thermophilus |
FRS | - |
Thermus thermophilus |
HSPC173 | - |
Thermus thermophilus |
L-Phenylalanyl-tRNA synthetase | - |
Thermus thermophilus |
Phenylalanine translase | - |
Thermus thermophilus |
Phenylalanine--tRNA ligase | - |
Thermus thermophilus |
Phenylalanine-tRNA synthetase | - |
Thermus thermophilus |
Phenylalanyl transfer ribonucleic acid synthetase | - |
Thermus thermophilus |
Phenylalanyl-transfer ribonucleate synthetase | - |
Thermus thermophilus |
Phenylalanyl-transfer RNA ligase | - |
Thermus thermophilus |
Phenylalanyl-transfer RNA synthetase | - |
Thermus thermophilus |
Phenylalanyl-tRNA ligase | - |
Thermus thermophilus |
Phenylalanyl-tRNA synthetase | - |
Thermus thermophilus |
PheRS | - |
Thermus thermophilus |
Synthetase, phenylalanyl-transfer ribonucleate | - |
Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Thermus thermophilus |