KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
Phe | - |
Phaseolus vulgaris | |
0.08 | - |
ATP | ATP | Phaseolus vulgaris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | optimal concentration is 10 mM | Phaseolus vulgaris | |
Mg2+ | optimal concentration is 20 mM | Phaseolus vulgaris | |
Mg2+ | both MgP2O7- and Mg2P2O7 participate in the pyrophosphorolysis of the aminoacyl adenylate | Phaseolus vulgaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
2 * 59000 (alpha) + 2 * 70000 (beta), PAGE under denaturing conditions | Phaseolus vulgaris |
70000 | - |
2 * 59000 (alpha) + 2 * 70000 (beta), PAGE under denaturing conditions | Phaseolus vulgaris |
260000 | - |
gel filtration | Phaseolus vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phaseolus vulgaris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Phaseolus vulgaris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Phaseolus vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-phenylalanine + tRNAPhe | tRNAs from E. coli and bean chloroplast are not aminoacylated | Phaseolus vulgaris | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 59000 (alpha) + 2 * 70000 (beta), PAGE under denaturing conditions | Phaseolus vulgaris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Phaseolus vulgaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
2-(morpholino)ethanesulfonic acid buffer or 2-(2-hydroxy-1,1-bis(hydroxymethyl)ethyl)aminoethanesulfonic acid buffer | Phaseolus vulgaris |
8.5 | - |
Tris/HCl buffer | Phaseolus vulgaris |