Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Methanothermobacter thermautotrophicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00133 | - |
tRNAGln(CUG) | wild type enzyme, pH and temperature not specified in the publication | Methanothermobacter thermautotrophicus | |
0.00361 | - |
tRNAGlu | wild type enzyme, pH and temperature not specified in the publication | Methanothermobacter thermautotrophicus | |
0.00539 | - |
tRNAGln(UUG) | wild type enzyme, pH and temperature not specified in the publication | Methanothermobacter thermautotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + tRNAGln(CUG) | Methanothermobacter thermautotrophicus | the enzyme shows a significant catalytic preference for tRNAGln(CUG) compared to the less active tRNAGln(UUG) | AMP + diphosphate + L-glutamyl-tRNAGln(CUG) | - |
? | |
ATP + L-glutamate + tRNAGln(UUG) | Methanothermobacter thermautotrophicus | the enzyme shows a significant catalytic preference for tRNAGln(CUG) compared to the less active tRNAGln(UUG) | AMP + diphosphate + L-glutamyl-tRNAGln(UUG) | - |
? | |
ATP + L-glutamate + tRNAGlu | Methanothermobacter thermautotrophicus | - |
AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + tRNAGln(CUG) | the enzyme shows a significant catalytic preference for tRNAGln(CUG) compared to the less active tRNAGln(UUG) | Methanothermobacter thermautotrophicus | AMP + diphosphate + L-glutamyl-tRNAGln(CUG) | - |
? | |
ATP + L-glutamate + tRNAGln(UUG) | the enzyme shows a significant catalytic preference for tRNAGln(CUG) compared to the less active tRNAGln(UUG) | Methanothermobacter thermautotrophicus | AMP + diphosphate + L-glutamyl-tRNAGln(UUG) | - |
? | |
ATP + L-glutamate + tRNAGlu | - |
Methanothermobacter thermautotrophicus | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GluRS | - |
Methanothermobacter thermautotrophicus |
Glutamyl-tRNA synthetase | - |
Methanothermobacter thermautotrophicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
tRNAGln(UUG) | wild type enzyme, pH and temperature not specified in the publication | Methanothermobacter thermautotrophicus | |
0.04 | - |
tRNAGlu | wild type enzyme, pH and temperature not specified in the publication | Methanothermobacter thermautotrophicus | |
0.041 | - |
tRNAGln(CUG) | wild type enzyme, pH and temperature not specified in the publication | Methanothermobacter thermautotrophicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Methanothermobacter thermautotrophicus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
tRNAGln(UUG) | wild type enzyme, pH and temperature not specified in the publication | Methanothermobacter thermautotrophicus | |
10 | - |
tRNAGlu | wild type enzyme, pH and temperature not specified in the publication | Methanothermobacter thermautotrophicus | |
310 | - |
tRNAGln(CUG) | wild type enzyme, pH and temperature not specified in the publication | Methanothermobacter thermautotrophicus |