Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | protein is not toxic when overproduced in Escherichia coli cells indicating that it does not catalyze the mischarging of Escherichia coli tRNAGln with l-Glu and that GluRS /tRNAGln recognition is species specific | Mycobacterium tuberculosis |
General Stability | Organism |
---|---|
Mycobacterium tuberculosis GluRS is significantly more sensitive than the Escherichia coli form to tryptic and chymotryptic limited proteolysis. Chymotrypsin-sensitive sites are found in the predicted tRNA stem contact domain next to the ATP binding site. Enzyme is fully protected from proteolysis by ATP and glutamol-AMP | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | competitive to ATP and L-glutamate, uncompetitive to tRNAGlu | Mycobacterium tuberculosis | |
glutamol-AMP | noncompetitive to ATP and L-glutamate | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
ATP | pH 7.3, 37°C | Mycobacterium tuberculosis | |
0.7 | - |
tRNAGlu | pH 7.3, 37°C | Mycobacterium tuberculosis | |
2.7 | - |
L-glutamate | pH 7.3, 37°C | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WFV9 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WFV9 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Mycobacterium tuberculosis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-glutamate + tRNAGlu = AMP + diphosphate + L-glutamyl-tRNAGlu | random binding of ATP and L-glutamate to the enzyme-tRNA complex | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + tRNAGlu | - |
Mycobacterium tuberculosis | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
ATP + L-glutamate + tRNAGlu | - |
Mycobacterium tuberculosis H37Rv | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.272 | - |
ATP | pH 7.3, 37°C | Mycobacterium tuberculosis | |
0.408 | - |
tRNAGlu | pH 7.3, 37°C | Mycobacterium tuberculosis | |
2.15 | - |
L-glutamate | pH 7.3, 37°C | Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
glutamol-AMP | substrate ATP, pH 7.3, 37°C | Mycobacterium tuberculosis | |
0.0039 | - |
glutamol-AMP | substrate L-glutamate, pH 7.3, 37°C | Mycobacterium tuberculosis | |
0.0039 | - |
glutamol-AMP | substrate tRNAGlu, pH 7.3, 37°C | Mycobacterium tuberculosis | |
0.027 | - |
diphosphate | substrate ATP, pH 7.3, 37°C | Mycobacterium tuberculosis | |
0.101 | - |
diphosphate | substrate L-glutamate, pH 7.3, 37°C | Mycobacterium tuberculosis |