Literature summary for 6.1.1.17 extracted from

Simader, H.; Hothorn, M.; Koehler, C.; Basquin, J.; Simos, G.; Suck, D.
Structural basis of yeast aminoacyl-tRNA synthetase complex formation revealed by crystal structures of two binary sub-complexes (2006), Nucleic Acids Res., 34, 3968-3979.

Search for more literature for 6.1.1.17

Activating Compound

Activating Compound	Comment	Organism	Structure
Arc1p	accessory protein Arc1p, SwissProt ID P46672	Saccharomyces cerevisiae
Arc1p-N	residues 1-122 of Arc1p, recombinantly expressed, the GluRS-NArc1p-N complex represents an unusual mode of interaction	Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type and mutant His-tagged GluRSs in Escherichia coli strain BL21(DE3)	Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant GluRS-N-Arc1p-N complex, hanging drop vapour diffusion method, 0.002 ml protein solution containing 15 mg/ml protein in 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, pH 7.2 with NaOH, is mixed with 0.002 ml reservoir solution containing 30-35% PEG 3350, 300-500 mM NaSCN, X-ray diffraction structure determination and analysis at 2.05 A resolution	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Saccharomyces cerevisiae	5737	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-glutamate + tRNAGlu	Saccharomyces cerevisiae	-	AMP + diphosphate + L-glutamyl-tRNAGlu	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	P46655	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged GluRS from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-glutamate + tRNAGlu	-	Saccharomyces cerevisiae	AMP + diphosphate + L-glutamyl-tRNAGlu	-	?
additional information	GluRS interacts with the accessory protein Arc1p, interaction mode and structure, overview	Saccharomyces cerevisiae	?	-	?

Synonyms

Synonyms	Comment	Organism
GluRS	-	Saccharomyces cerevisiae
Glutamyl-tRNA synthetase	-	Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	assay at	Saccharomyces cerevisiae

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)