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Literature summary for 6.1.1.17 extracted from

  • Proulx, M.; Lapointe, J.
    Purification of glutamyl-tRNA synthetase from Bacillus subtilis (1985), Methods Enzymol., 113, 50-54.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
Trp-
-
Bacillus subtilis 168
-
Trp-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.064
-
-
Bacillus subtilis

Storage Stability

Storage Stability Organism
-20°C, 10 mM Tris-HCl, pH 8.0, 1 mM MgCl2, 20 mM 2-mercaptoethanol, 0.1 mM PMSF, 50% glycerol, stable for several months Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + tRNAGlu
-
Bacillus subtilis AMP + diphosphate + L-glutamyl-tRNAGlu
-
?
ATP + L-glutamate + tRNAGlu
-
Bacillus subtilis 168 AMP + diphosphate + L-glutamyl-tRNAGlu
-
?