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Literature summary for 6.1.1.17 extracted from

  • Lapointe, J.; Soll, D.
    Glutamyl transfer ribonucleic acid synthetase of Escherichia coli (1972), J. Biol. Chem., 247, 4982-4985.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00012
-
tRNAGlu 56000 MW subunit alone Escherichia coli
0.0003
-
tRNAGlu 56000 MW subunit and 46000 MW subunit Escherichia coli
0.027
-
Glu 56000 MW subunit and 46000 MW subunit Escherichia coli
0.086
-
Glu 56000 MW subunit alone Escherichia coli
0.1
-
ATP ATP, , 56000 MW subunit and 46000 MW subunit Escherichia coli
0.25
-
ATP 56000 MW subunit alone Escherichia coli
0.32
-
ATP ATP-diphosphate exchange Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
1 * 56000 (catalytic subunit) + 1 * 46000 (no detectable enzymatic activity, protective function), SDS-PAGE Escherichia coli
56000
-
1 * 56000 (catalytic subunit) + 1 * 46000 (no detectable enzymatic activity, protective function), SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + tRNAGlu
-
Escherichia coli AMP + diphosphate + L-glutamyl-tRNAGlu
-
?

Subunits

Subunits Comment Organism
dimer 1 * 56000 (catalytic subunit) + 1 * 46000 (no detectable enzymatic activity, protective function), SDS-PAGE Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
tRNAGlu
-
Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
glutamyl-tRNA formation Escherichia coli