Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E354Q | the mutant has decreased kcat and Km values leading to an overall decrease in kcat/Km by 6.8fold relative to the wild type enzyme | Escherichia coli |
E354Q/R427A | the mutant has a decreased kcat and an increased Km leading to an overall decrease in kcat/Km by 22fold relative to the wild type enzyme | Escherichia coli |
H297A | the mutant has a decreased kcat and Km values leading to an overall decrease in kcat/Km by 3.4fold relative to the wild type enzyme | Escherichia coli |
H404A/R42A | the mutant has a decreased kcat and an increased Km leading to an overall decrease in kcat/Km by 209fold relative to the wild type enzyme | Escherichia coli |
H40A | the mutant has a decreased kcat and an increased Km leading to an overall decrease in kcat/Km by 204fold relative to the wild type enzyme | Escherichia coli |
M294A | the mutant has decreased kcat and Km values leading to an overall decrease in kcat/Km by 3.7fold relative to the wild type enzyme | Escherichia coli |
M294A/H297A | the mutant has a decreased kcat and Km values leading to an overall decrease in kcat/Km by 8.1fold relative to the wild type enzyme | Escherichia coli |
M294A/R427A | the mutant has a decreased kcat and an increased Km leading to an overall decrease in kcat/Km by 370fold relative to the wild type enzyme | Escherichia coli |
R427A | the mutant has a decreased kcat and an increased Km leading to an overall decrease in kcat/Km by 291fold relative to the wild type enzyme | Escherichia coli |
R42A | the mutant has a decreased kcat and an increased Km leading to an overall decrease in kcat/Km by 1.5fold relative to the wild type enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00047 | - |
L-cysteine | mutant enzyme M294A, pH and temperature not specified in the publication | Escherichia coli | |
0.00052 | - |
L-cysteine | mutant enzyme E354Q, pH and temperature not specified in the publication | Escherichia coli | |
0.00055 | - |
L-cysteine | mutant enzyme H297A, pH and temperature not specified in the publication | Escherichia coli | |
0.00066 | - |
L-cysteine | mutant enzyme M294A/H297A, pH and temperature not specified in the publication | Escherichia coli | |
0.0012 | - |
L-cysteine | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli | |
0.0018 | - |
L-cysteine | mutant enzyme R42A, pH and temperature not specified in the publication | Escherichia coli | |
0.002 | - |
L-cysteine | mutant enzyme H40A, pH and temperature not specified in the publication | Escherichia coli | |
0.0075 | - |
L-cysteine | mutant enzyme R427A, pH and temperature not specified in the publication | Escherichia coli | |
0.0076 | - |
L-cysteine | mutant enzyme M294A/R427A, pH and temperature not specified in the publication | Escherichia coli | |
0.01 | - |
L-cysteine | mutant enzyme E354Q/R427A, pH and temperature not specified in the publication | Escherichia coli | |
2.4 | - |
L-cysteine | mutant enzyme H40A/R42A, pH and temperature not specified in the publication | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P21888 | - |
- |
Purification (Comment) | Organism |
---|---|
Talon resin column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-cysteine + tRNACys | - |
Escherichia coli | AMP + diphosphate + L-cysteinyl-tRNACys | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CysRS | - |
Escherichia coli |
cysteinyl tRNA synthetase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
L-cysteine | mutant enzyme H40A/R42A, pH and temperature not specified in the publication | Escherichia coli | |
0.0093 | - |
L-cysteine | mutant enzyme H40A, pH and temperature not specified in the publication | Escherichia coli | |
0.02 | - |
L-cysteine | mutant enzyme M294A/R427A, pH and temperature not specified in the publication | Escherichia coli | |
0.027 | - |
L-cysteine | mutant enzyme R427A, pH and temperature not specified in the publication | Escherichia coli | |
0.082 | - |
L-cysteine | mutant enzyme E354Q, pH and temperature not specified in the publication | Escherichia coli | |
0.088 | - |
L-cysteine | mutant enzyme M294A/H297A, pH and temperature not specified in the publication | Escherichia coli | |
0.14 | - |
L-cysteine | mutant enzyme M294A, pH and temperature not specified in the publication | Escherichia coli | |
0.16 | - |
L-cysteine | mutant enzyme H297A, pH and temperature not specified in the publication | Escherichia coli | |
0.49 | - |
L-cysteine | mutant enzyme E354Q/R427A, pH and temperature not specified in the publication | Escherichia coli | |
1.1 | - |
L-cysteine | mutant enzyme R42A, pH and temperature not specified in the publication | Escherichia coli | |
1.2 | - |
L-cysteine | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
L-cysteine | mutant enzyme M294A/R427A, pH and temperature not specified in the publication | Escherichia coli | |
3.8 | - |
L-cysteine | mutant enzyme R427A, pH and temperature not specified in the publication | Escherichia coli | |
5.3 | - |
L-cysteine | mutant enzyme H40A/R42A, pH and temperature not specified in the publication | Escherichia coli | |
5.4 | - |
L-cysteine | mutant enzyme H40A, pH and temperature not specified in the publication | Escherichia coli | |
50 | - |
L-cysteine | mutant enzyme E354Q/R427A, pH and temperature not specified in the publication | Escherichia coli | |
140 | - |
L-cysteine | mutant enzyme M294A/H297A, pH and temperature not specified in the publication | Escherichia coli | |
160 | - |
L-cysteine | mutant enzyme E354Q, pH and temperature not specified in the publication | Escherichia coli | |
300 | - |
L-cysteine | mutant enzyme M294A, pH and temperature not specified in the publication | Escherichia coli | |
320 | - |
L-cysteine | mutant enzyme H297A, pH and temperature not specified in the publication | Escherichia coli | |
750 | - |
L-cysteine | mutant enzyme R42A, pH and temperature not specified in the publication | Escherichia coli | |
1000 | - |
L-cysteine | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli |