KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | thermodynamics, single turnover and burst kinetics of CysRS, steady-state and transient kinetic analyses of class I CysRS, the enzyme is rate-limited by release of aminoacyl-tRNA, recombinant His-tagged enzyme, overview | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-cysteine + tRNACys | Escherichia coli | - |
AMP + diphosphate + L-cysteinyl-tRNACys | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P21888 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-cysteine + tRNACys = AMP + diphosphate + L-cysteinyl-tRNACys | the class I tRNA synthetase CysRS is rate-limited by release of aminoacyl-tRNA, class I synthetases share two signature motifs, HIGH and KMSKS, and build their active site by the ubiquitous Rossmann nucleotide-binding fold, the tight aminoacyl-tRNA product binding by class I enzymes correlates with the ability of EF-Tu to form a ternary complex with class I but not class II synthetases, and the further capacity of this protein to enhance the rate of aminoacylation by class I synthetases | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-cysteine + tRNACys | - |
Escherichia coli | AMP + diphosphate + L-cysteinyl-tRNACys | - |
? | |
ATP + L-cysteine + tRNACys | the enzyme is rate-limited by release of aminoacyl-tRNA | Escherichia coli | AMP + diphosphate + L-cysteinyl-tRNACys | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme forms a class I ternary complex with EF-Tu, overview | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
class I CysRS | - |
Escherichia coli |
class I cysteinyl-tRNA synthetase | - |
Escherichia coli |
CysRS | - |
Escherichia coli |
Cysteinyl-tRNA synthetase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
L-cysteine | steady-state | Escherichia coli | |
2.9 | - |
tRNACys | steady-state | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |