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Literature summary for 6.1.1.16 extracted from

  • Zhang, C.M.; Perona, J.J.; Ryu, K.; Francklyn, C.; Hou, Y.M.
    Distinct kinetic mechanisms of the two classes of aminoacyl-tRNA synthetases (2006), J. Mol. Biol., 361, 300-311.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information thermodynamics, single turnover and burst kinetics of CysRS, steady-state and transient kinetic analyses of class I CysRS, the enzyme is rate-limited by release of aminoacyl-tRNA, recombinant His-tagged enzyme, overview Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-cysteine + tRNACys Escherichia coli
-
AMP + diphosphate + L-cysteinyl-tRNACys
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P21888
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + L-cysteine + tRNACys = AMP + diphosphate + L-cysteinyl-tRNACys the class I tRNA synthetase CysRS is rate-limited by release of aminoacyl-tRNA, class I synthetases share two signature motifs, HIGH and KMSKS, and build their active site by the ubiquitous Rossmann nucleotide-binding fold, the tight aminoacyl-tRNA product binding by class I enzymes correlates with the ability of EF-Tu to form a ternary complex with class I but not class II synthetases, and the further capacity of this protein to enhance the rate of aminoacylation by class I synthetases Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-cysteine + tRNACys
-
Escherichia coli AMP + diphosphate + L-cysteinyl-tRNACys
-
?
ATP + L-cysteine + tRNACys the enzyme is rate-limited by release of aminoacyl-tRNA Escherichia coli AMP + diphosphate + L-cysteinyl-tRNACys
-
?

Subunits

Subunits Comment Organism
More the enzyme forms a class I ternary complex with EF-Tu, overview Escherichia coli

Synonyms

Synonyms Comment Organism
class I CysRS
-
Escherichia coli
class I cysteinyl-tRNA synthetase
-
Escherichia coli
CysRS
-
Escherichia coli
Cysteinyl-tRNA synthetase
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5
-
L-cysteine steady-state Escherichia coli
2.9
-
tRNACys steady-state Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
Escherichia coli