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Literature summary for 6.1.1.16 extracted from
- Purification and properties of cysteinyl-tRNA synthetase from rabbit liver (1998), Biochimie, 80, 579-590.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
L-cysteine | pH 7.5, 25°C | Oryctolagus cuniculus |  |
| 0.08 | - |
tRNACys | pH 7.5, 25°C | Oryctolagus cuniculus | |
| 0.8 | - |
ATP | pH 7.5, 25°C | Oryctolagus cuniculus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | required, optimal cocentration is 150 mM | Oryctolagus cuniculus | |
| Mg2+ | dependent on, optimal concentration is 5 mM | Oryctolagus cuniculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 86000 | - |
x * 92000, enzyme form I, SDS-PAGE, x * 86000, enzyme form II, SDS-PAGE | Oryctolagus cuniculus |
| 92000 | - |
x * 92000, enzyme form I, SDS-PAGE, x * 86000, enzyme form II, SDS-PAGE | Oryctolagus cuniculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-cysteine + tRNACys | Oryctolagus cuniculus | - |
AMP + diphosphate + L-cysteinyl-tRNACys | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 8300fold from liver, 2 different forms, to homogeneity | Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.22 | - |
purified enzyme | Oryctolagus cuniculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50% glycerol, 1 mM DTE, at least 6 months, no loss of activity | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-cysteine + tRNACys | - |
Oryctolagus cuniculus | AMP + diphosphate + L-cysteinyl-tRNACys | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 92000, enzyme form I, SDS-PAGE, x * 86000, enzyme form II, SDS-PAGE | Oryctolagus cuniculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CRS | - |
Oryctolagus cuniculus |
| CysRS | - |
Oryctolagus cuniculus |
| Cysteine translase | - |
Oryctolagus cuniculus |
| Cysteine--tRNA ligase | - |
Oryctolagus cuniculus |
| Cysteinyl-transfer ribonucleate synthetase | - |
Oryctolagus cuniculus |
| Cysteinyl-transfer RNA synthetase | - |
Oryctolagus cuniculus |
| Cysteinyl-tRNA synthetase | - |
Oryctolagus cuniculus |
| Synthetase, cysteinyl-transfer ribonucleate | - |
Oryctolagus cuniculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Oryctolagus cuniculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | dependent on, optimal concentration is 3 mM | Oryctolagus cuniculus | |
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