Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.16 extracted from

  • Pan, F.; Yu, T.C.; Duh, G.M.; Lee, H.H.
    Specificity and kinetic properties of the cysteinyl-tRNA synthetase from rat liver (1976), J. Chin. Biochem. Soc., 5, 45-56.
No PubMed abstract available
Search for more literature for 6.1.1.16

Inhibitors

Inhibitors Comment Organism Structure
2'-deoxy-ATP
-
 Rattus norvegicus
ADP noncompetitive Rattus norvegicus
alpha,beta-CH2-ATP competitive Rattus norvegicus
AMP noncompetitive Rattus norvegicus
ATP at high concentrations Rattus norvegicus
Ba2+ 10 mM, 43% inhibition in presence of 10 mM Mg2+, no activation in absence of Mg2+ Rattus norvegicus
Ca2+ 10 mM, 28% inhibition in presence of 10 mM Mg2+, no activation in absence of Mg2+ Rattus norvegicus
Co2+ 10 mM, complete inhibition in presence of 10 mM Mg2+, no activation in absence of Mg2+ Rattus norvegicus
Cu2+ 10 mM, complete inhibition in presence of 10 mM Mg2+, no activation in absence of Mg2+ Rattus norvegicus
cysteamine competitive Rattus norvegicus
cysteine at high concentrations Rattus norvegicus
D-cysteine weak, non-competitive Rattus norvegicus
diphosphate at high concentrations Rattus norvegicus
GTP noncompetitive Rattus norvegicus
Hg2+ 10 mM, complete inhibition in presence of 10 mM Mg2+, no activation in absence of Mg2+ Rattus norvegicus
iodoacetamide partially Rattus norvegicus
L-homocysteine
-
 Rattus norvegicus
S-methyl-L-cysteine competitive Rattus norvegicus
Sodium tripolyphosphate
-
 Rattus norvegicus
Zn2+ 10 mM, complete inhibition in presence of 10 mM Mg2+, no activation in absence of Mg2+ Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 Cys
-
 Rattus norvegicus
0.16
-
 diphosphate
-
 Rattus norvegicus
1.8
-
 ATP
-
 Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimal concentration: 10 mM Rattus norvegicus
Mg2+ inhibition above 15 mM Rattus norvegicus
Mg2+ required for ATP-diphosphate exchange Rattus norvegicus
Mg2+ Km: 3.6 mM Rattus norvegicus
Mn2+ can partially replace Mg2+ in activation of cysteine, optimal concentration is 5 mM Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-cysteine + tRNACys
-
 Rattus norvegicus AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Rattus norvegicus