Literature summary for 6.1.1.15 extracted from

Son, J.; Lee, E.H.; Park, M.; Kim, J.H.; Kim, J.; Kim, S.; Jeon, Y.H.; Hwang, K.Y.
Conformational changes in human prolyl-tRNA synthetase upon binding of the substrates proline and ATP and the inhibitor halofuginone (2013), Acta Crystallogr. Sect. D, 69, 2136-2145.

Search for more literature for 6.1.1.15

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta 2 (DE3) pLysS cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
complexed with ATP and L-proline or halofuginone, hanging drop vapor diffusion method, using 0.1 M sodium cacodylate, 1.0 M sodium citrate dibasic trihydrate pH 6.5	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
F1097A	the mutant shows strongly decreased aminoacylation activity compared to the wild type enzyme	Homo sapiens
F1097W	the mutant shows increased aminoacylation activity compared to the wild type enzyme	Homo sapiens
R1152K	the mutant shows increased aminoacylation activity compared to the wild type enzyme	Homo sapiens
R1152L	the mutant shows strongly decreased aminoacylation activity compared to the wild type enzyme	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
halofuginone	strong inhibition at 0.001 mM	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-proline + tRNAPro	Homo sapiens	-	AMP + diphosphate + L-prolyl-tRNAPro	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P07814	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA affinity column chromatography and Superdex 200 gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-proline + tRNAPro	-	Homo sapiens	AMP + diphosphate + L-prolyl-tRNAPro	-	?

Synonyms

Synonyms	Comment	Organism
Prolyl-tRNA synthetase	-	Homo sapiens
PRS	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens

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Release 2023.1 (January 2023)