Cloned (Comment) | Organism |
---|---|
expression as His-tagged enzyme in Escherichia coli | Methanothermobacter thermautotrophicus |
Crystallization (Comment) | Organism |
---|---|
vapour diffusion method, equal volumes of 3.8 mg/ml protein solution and precipitant solution containing 20 mM Tris, pH 7.5, 5 mM 2-mercaptoethanol, 50-250 mM MgCl2, 20°C, X-ray diffraction structure determination at 2.55-3.1 A, and analysis | Methanothermobacter thermautotrophicus |
vapour-diffusion method, protein solution is mixed with an equal volume of well solution containing 0.2 M ammonium sulfate, 20-30% PEG 4000, 5 mM 2-mercaptoethanol, 0.1 M sodium acetate, pH 4.5, 12-25°C, a few days, X-ray diffraction structure determination at 3.2 A resolution, and analysis | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cysteinyl-sulfamoyl-adenylate | i.e. Cys-AMS, intermediate analogue, competitive inhibition | Methanothermobacter thermautotrophicus | |
prolyl-sulfamoyl-adenylate | i.e. Pro-AMS, intermediate analogue, competitive inhibition | Methanothermobacter thermautotrophicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
L-cysteine | ATP-diphosphate exchange, pH 7.5, 35°C | Methanothermobacter thermautotrophicus | |
0.067 | - |
L-proline | ATP-diphosphate exchange reaction, pH 7.5, 35°C | Methanothermobacter thermautotrophicus | |
0.09 | - |
L-cysteine | ATP-diphosphate exchange reaction, pH 7.5, 35°C | Methanocaldococcus jannaschii | |
0.285 | - |
L-proline | ATP-diphosphate exchange reaction, pH 7.5, 35°C | Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | chelated by 4 cysteine residues in the C-terminus | Methanothermobacter thermautotrophicus | |
Zn2+ | chelated by 4 cysteine residues in the C-terminus | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-proline + tRNAPro | Methanothermobacter thermautotrophicus | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | Methanocaldococcus jannaschii | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Methanothermobacter thermautotrophicus | - |
gene proS | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli | Methanothermobacter thermautotrophicus |
recombinant His-tagged enzyme from expression in Escherichia coli | Methanocaldococcus jannaschii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-proline + tRNAPro = AMP + diphosphate + L-prolyl-tRNAPro | dual substrate specificity | Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-cysteine + tRNACys | - |
Methanothermobacter thermautotrophicus | AMP + diphosphate + L-cysteinyl-tRNACys | - |
? | |
ATP + L-proline + tRNAPro | - |
Methanothermobacter thermautotrophicus | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | - |
Methanocaldococcus jannaschii | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
additional information | the enzyme also performs the ATP-diphosphate exchange reaction | Methanothermobacter thermautotrophicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Global RNA synthesis factor | - |
Methanothermobacter thermautotrophicus |
Global RNA synthesis factor | - |
Methanocaldococcus jannaschii |
Pro-tRNA synthetase | - |
Methanothermobacter thermautotrophicus |
Pro-tRNA synthetase | - |
Methanocaldococcus jannaschii |
Proline translase | - |
Methanothermobacter thermautotrophicus |
Proline translase | - |
Methanocaldococcus jannaschii |
Proline--tRNA ligase | - |
Methanothermobacter thermautotrophicus |
Proline--tRNA ligase | - |
Methanocaldococcus jannaschii |
Prolyl RNA synthetase | - |
Methanothermobacter thermautotrophicus |
Prolyl RNA synthetase | - |
Methanocaldococcus jannaschii |
Prolyl-transfer ribonucleate synthetase | - |
Methanothermobacter thermautotrophicus |
Prolyl-transfer ribonucleate synthetase | - |
Methanocaldococcus jannaschii |
Prolyl-transfer ribonucleic acid synthetase | - |
Methanothermobacter thermautotrophicus |
Prolyl-transfer ribonucleic acid synthetase | - |
Methanocaldococcus jannaschii |
Prolyl-transfer RNA synthetase | - |
Methanothermobacter thermautotrophicus |
Prolyl-transfer RNA synthetase | - |
Methanocaldococcus jannaschii |
Prolyl-tRNA synthetase | - |
Methanothermobacter thermautotrophicus |
Prolyl-tRNA synthetase | - |
Methanocaldococcus jannaschii |
ProRS | - |
Methanothermobacter thermautotrophicus |
ProRS | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Methanothermobacter thermautotrophicus |
35 | - |
assay at | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Methanothermobacter thermautotrophicus |
7.5 | - |
assay at | Methanocaldococcus jannaschii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Methanothermobacter thermautotrophicus | |
ATP | - |
Methanocaldococcus jannaschii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000025 | - |
cysteinyl-sulfamoyl-adenylate | versus L-proline, pH 7.5, 35°C | Methanothermobacter thermautotrophicus | |
0.00005 | - |
prolyl-sulfamoyl-adenylate | versus L-proline, pH 7.5, 35°C | Methanothermobacter thermautotrophicus |