Activating Compound | Comment | Organism | Structure |
---|---|---|---|
tRNA | stimulates l-cysteine activation 2-3fold | Methanocaldococcus jannaschii |
Cloned (Comment) | Organism |
---|---|
gene proS, overexpression in Escherichia coli | Methanocaldococcus jannaschii |
Crystallization (Comment) | Organism |
---|---|
crystal structure analysis | Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
E103A | abolished activity with L-proline | Methanocaldococcus jannaschii |
additional information | the dual-specific enzyme is sufficient for Cys-tRNACys production in a mutant disrupted in the gene encoing the canonical CysRS | Methanococcus maripaludis |
P100A | highly reduced activity with L-cysteine | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
proline analogues | - |
Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-cysteine + tRNAPro | Methanocaldococcus jannaschii | two-step reaction | AMP + diphosphate + L-cysteinyl-tRNAPro | - |
r | |
ATP + L-proline + tRNAPro | Thermus thermophilus | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | Giardia intestinalis | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | Methanocaldococcus jannaschii | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
r | |
ATP + L-proline + tRNAPro | Methanococcus maripaludis | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
additional information | Methanocaldococcus jannaschii | phylogenetic analysis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Giardia intestinalis | - |
enzyme with dual substrate specificity | - |
Methanocaldococcus jannaschii | - |
enzyme with dual substrate specificity | - |
Methanococcus maripaludis | - |
enzyme with dual substrate specificity | - |
Thermus thermophilus | - |
enzyme with dual substrate specificity | - |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli | Methanocaldococcus jannaschii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-proline + tRNAPro = AMP + diphosphate + L-prolyl-tRNAPro | enzyme with dual substrate specificity which utilizes L-proline and L-cysteine as substrates, discriminating mechanism | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-cysteine + tRNAPro | two-step reaction | Methanocaldococcus jannaschii | AMP + diphosphate + L-cysteinyl-tRNAPro | - |
r | |
ATP + L-cysteine + tRNAPro | dual-specificity enzyme | Giardia intestinalis | AMP + diphosphate + L-cysteinyl-tRNAPro | - |
? | |
ATP + L-cysteine + tRNAPro | dual-specificity enzyme | Methanococcus maripaludis | AMP + diphosphate + L-cysteinyl-tRNAPro | - |
? | |
ATP + L-cysteine + tRNAPro | dual-specificity enzyme, enzyme contains a discrete cysteine binding pocket | Thermus thermophilus | AMP + diphosphate + L-cysteinyl-tRNAPro | - |
? | |
ATP + L-cysteine + tRNAPro | two-step reaction, both steps are dependent on tRNACys | Methanocaldococcus jannaschii | AMP + diphosphate + L-cysteinyl-tRNAPro | - |
r | |
ATP + L-proline + tRNAPro | - |
Thermus thermophilus | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | - |
Giardia intestinalis | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | - |
Methanocaldococcus jannaschii | AMP + diphosphate + L-prolyl-tRNAPro | - |
r | |
ATP + L-proline + tRNAPro | - |
Methanococcus maripaludis | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | two-step reaction | Methanocaldococcus jannaschii | AMP + diphosphate + L-prolyl-tRNAPro | - |
r | |
additional information | the enzyme also performs the ATP-diphosphate exchange reaction, the enzyme possesses a pre- and post-editing mechanism for alanine | Methanocaldococcus jannaschii | ? | - |
? | |
additional information | phylogenetic analysis | Methanocaldococcus jannaschii | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Global RNA synthesis factor | - |
Thermus thermophilus |
Global RNA synthesis factor | - |
Giardia intestinalis |
Global RNA synthesis factor | - |
Methanocaldococcus jannaschii |
Global RNA synthesis factor | - |
Methanococcus maripaludis |
Pro-tRNA synthetase | - |
Thermus thermophilus |
Pro-tRNA synthetase | - |
Giardia intestinalis |
Pro-tRNA synthetase | - |
Methanocaldococcus jannaschii |
Pro-tRNA synthetase | - |
Methanococcus maripaludis |
Proline translase | - |
Thermus thermophilus |
Proline translase | - |
Giardia intestinalis |
Proline translase | - |
Methanocaldococcus jannaschii |
Proline translase | - |
Methanococcus maripaludis |
Proline--tRNA ligase | - |
Thermus thermophilus |
Proline--tRNA ligase | - |
Giardia intestinalis |
Proline--tRNA ligase | - |
Methanocaldococcus jannaschii |
Proline--tRNA ligase | - |
Methanococcus maripaludis |
Prolyl RNA synthetase | - |
Thermus thermophilus |
Prolyl RNA synthetase | - |
Giardia intestinalis |
Prolyl RNA synthetase | - |
Methanocaldococcus jannaschii |
Prolyl RNA synthetase | - |
Methanococcus maripaludis |
Prolyl-transfer ribonucleate synthetase | - |
Thermus thermophilus |
Prolyl-transfer ribonucleate synthetase | - |
Giardia intestinalis |
Prolyl-transfer ribonucleate synthetase | - |
Methanocaldococcus jannaschii |
Prolyl-transfer ribonucleate synthetase | - |
Methanococcus maripaludis |
Prolyl-transfer ribonucleic acid synthetase | - |
Thermus thermophilus |
Prolyl-transfer ribonucleic acid synthetase | - |
Giardia intestinalis |
Prolyl-transfer ribonucleic acid synthetase | - |
Methanocaldococcus jannaschii |
Prolyl-transfer ribonucleic acid synthetase | - |
Methanococcus maripaludis |
Prolyl-transfer RNA synthetase | - |
Thermus thermophilus |
Prolyl-transfer RNA synthetase | - |
Giardia intestinalis |
Prolyl-transfer RNA synthetase | - |
Methanocaldococcus jannaschii |
Prolyl-transfer RNA synthetase | - |
Methanococcus maripaludis |
Prolyl-tRNA synthetase | - |
Thermus thermophilus |
Prolyl-tRNA synthetase | - |
Giardia intestinalis |
Prolyl-tRNA synthetase | - |
Methanocaldococcus jannaschii |
Prolyl-tRNA synthetase | - |
Methanococcus maripaludis |
ProRS | - |
Thermus thermophilus |
ProRS | - |
Giardia intestinalis |
ProRS | - |
Methanocaldococcus jannaschii |
ProRS | - |
Methanococcus maripaludis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Thermus thermophilus | |
ATP | - |
Giardia intestinalis | |
ATP | - |
Methanocaldococcus jannaschii | |
ATP | - |
Methanococcus maripaludis |