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Literature summary for 6.1.1.15 extracted from

  • Jacquin-Becker, C.; Ahel, I.; Ambrogelly, A.; Ruan, B.; Soll, D.; Stathopoulos, C.
    Cysteinyl-tRNA formation and prolyl-tRNA synthetase (2002), FEBS Lett., 514, 34-36.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
tRNA stimulates l-cysteine activation 2-3fold Methanocaldococcus jannaschii

Cloned(Commentary)

Cloned (Comment) Organism
gene proS, overexpression in Escherichia coli Methanocaldococcus jannaschii

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure analysis Thermus thermophilus

Protein Variants

Protein Variants Comment Organism
E103A abolished activity with L-proline Methanocaldococcus jannaschii
additional information the dual-specific enzyme is sufficient for Cys-tRNACys production in a mutant disrupted in the gene encoing the canonical CysRS Methanococcus maripaludis
P100A highly reduced activity with L-cysteine Methanocaldococcus jannaschii

Inhibitors

Inhibitors Comment Organism Structure
proline analogues
-
Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-cysteine + tRNAPro Methanocaldococcus jannaschii two-step reaction AMP + diphosphate + L-cysteinyl-tRNAPro
-
r
ATP + L-proline + tRNAPro Thermus thermophilus
-
AMP + diphosphate + L-prolyl-tRNAPro
-
?
ATP + L-proline + tRNAPro Giardia intestinalis
-
AMP + diphosphate + L-prolyl-tRNAPro
-
?
ATP + L-proline + tRNAPro Methanocaldococcus jannaschii
-
AMP + diphosphate + L-prolyl-tRNAPro
-
r
ATP + L-proline + tRNAPro Methanococcus maripaludis
-
AMP + diphosphate + L-prolyl-tRNAPro
-
?
additional information Methanocaldococcus jannaschii phylogenetic analysis ?
-
?

Organism

Organism UniProt Comment Textmining
Giardia intestinalis
-
enzyme with dual substrate specificity
-
Methanocaldococcus jannaschii
-
enzyme with dual substrate specificity
-
Methanococcus maripaludis
-
enzyme with dual substrate specificity
-
Thermus thermophilus
-
enzyme with dual substrate specificity
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from Escherichia coli Methanocaldococcus jannaschii

Reaction

Reaction Comment Organism Reaction ID
ATP + L-proline + tRNAPro = AMP + diphosphate + L-prolyl-tRNAPro enzyme with dual substrate specificity which utilizes L-proline and L-cysteine as substrates, discriminating mechanism Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-cysteine + tRNAPro two-step reaction Methanocaldococcus jannaschii AMP + diphosphate + L-cysteinyl-tRNAPro
-
r
ATP + L-cysteine + tRNAPro dual-specificity enzyme Giardia intestinalis AMP + diphosphate + L-cysteinyl-tRNAPro
-
?
ATP + L-cysteine + tRNAPro dual-specificity enzyme Methanococcus maripaludis AMP + diphosphate + L-cysteinyl-tRNAPro
-
?
ATP + L-cysteine + tRNAPro dual-specificity enzyme, enzyme contains a discrete cysteine binding pocket Thermus thermophilus AMP + diphosphate + L-cysteinyl-tRNAPro
-
?
ATP + L-cysteine + tRNAPro two-step reaction, both steps are dependent on tRNACys Methanocaldococcus jannaschii AMP + diphosphate + L-cysteinyl-tRNAPro
-
r
ATP + L-proline + tRNAPro
-
Thermus thermophilus AMP + diphosphate + L-prolyl-tRNAPro
-
?
ATP + L-proline + tRNAPro
-
Giardia intestinalis AMP + diphosphate + L-prolyl-tRNAPro
-
?
ATP + L-proline + tRNAPro
-
Methanocaldococcus jannaschii AMP + diphosphate + L-prolyl-tRNAPro
-
r
ATP + L-proline + tRNAPro
-
Methanococcus maripaludis AMP + diphosphate + L-prolyl-tRNAPro
-
?
ATP + L-proline + tRNAPro two-step reaction Methanocaldococcus jannaschii AMP + diphosphate + L-prolyl-tRNAPro
-
r
additional information the enzyme also performs the ATP-diphosphate exchange reaction, the enzyme possesses a pre- and post-editing mechanism for alanine Methanocaldococcus jannaschii ?
-
?
additional information phylogenetic analysis Methanocaldococcus jannaschii ?
-
?

Synonyms

Synonyms Comment Organism
Global RNA synthesis factor
-
Thermus thermophilus
Global RNA synthesis factor
-
Giardia intestinalis
Global RNA synthesis factor
-
Methanocaldococcus jannaschii
Global RNA synthesis factor
-
Methanococcus maripaludis
Pro-tRNA synthetase
-
Thermus thermophilus
Pro-tRNA synthetase
-
Giardia intestinalis
Pro-tRNA synthetase
-
Methanocaldococcus jannaschii
Pro-tRNA synthetase
-
Methanococcus maripaludis
Proline translase
-
Thermus thermophilus
Proline translase
-
Giardia intestinalis
Proline translase
-
Methanocaldococcus jannaschii
Proline translase
-
Methanococcus maripaludis
Proline--tRNA ligase
-
Thermus thermophilus
Proline--tRNA ligase
-
Giardia intestinalis
Proline--tRNA ligase
-
Methanocaldococcus jannaschii
Proline--tRNA ligase
-
Methanococcus maripaludis
Prolyl RNA synthetase
-
Thermus thermophilus
Prolyl RNA synthetase
-
Giardia intestinalis
Prolyl RNA synthetase
-
Methanocaldococcus jannaschii
Prolyl RNA synthetase
-
Methanococcus maripaludis
Prolyl-transfer ribonucleate synthetase
-
Thermus thermophilus
Prolyl-transfer ribonucleate synthetase
-
Giardia intestinalis
Prolyl-transfer ribonucleate synthetase
-
Methanocaldococcus jannaschii
Prolyl-transfer ribonucleate synthetase
-
Methanococcus maripaludis
Prolyl-transfer ribonucleic acid synthetase
-
Thermus thermophilus
Prolyl-transfer ribonucleic acid synthetase
-
Giardia intestinalis
Prolyl-transfer ribonucleic acid synthetase
-
Methanocaldococcus jannaschii
Prolyl-transfer ribonucleic acid synthetase
-
Methanococcus maripaludis
Prolyl-transfer RNA synthetase
-
Thermus thermophilus
Prolyl-transfer RNA synthetase
-
Giardia intestinalis
Prolyl-transfer RNA synthetase
-
Methanocaldococcus jannaschii
Prolyl-transfer RNA synthetase
-
Methanococcus maripaludis
Prolyl-tRNA synthetase
-
Thermus thermophilus
Prolyl-tRNA synthetase
-
Giardia intestinalis
Prolyl-tRNA synthetase
-
Methanocaldococcus jannaschii
Prolyl-tRNA synthetase
-
Methanococcus maripaludis
ProRS
-
Thermus thermophilus
ProRS
-
Giardia intestinalis
ProRS
-
Methanocaldococcus jannaschii
ProRS
-
Methanococcus maripaludis

Cofactor

Cofactor Comment Organism Structure
ATP
-
Thermus thermophilus
ATP
-
Giardia intestinalis
ATP
-
Methanocaldococcus jannaschii
ATP
-
Methanococcus maripaludis