General Stability | Organism |
---|---|
L-azetidine-2-carboxylic acid, cis(exo)-3,4-methano-L-proline, 3,4-dehydro-DL-proline, L-thiazolidine-4-carboxylic acid, N-methylglycine, N-methyl-L-alanine or trans-3-hydroxy-L-proline protect against inactivation at 70°C and 40°C | Phaseolus vulgaris |
L-azetidine-2-carboxylic acid, cis(exo)-3,4-methano-L-proline, 3,4-dehydro-DL-proline, L-thiazolidine-4-carboxylic acid, N-methylglycine, N-methyl-L-alanine or trans-3-hydroxy-L-proline protect against inactivation at 70°C and 40°C | Delonix regia |
Pro or ATP markedly stabilizes against heat inactivation | Phaseolus vulgaris |
Pro or ATP markedly stabilizes against heat inactivation | Delonix regia |
Pro or ATP markedly stabilizes against heat inactivation | Parkinsonia aculeata |
several analogues of Pro protect against thermal inactivation | Phaseolus vulgaris |
several analogues of Pro protect against thermal inactivation | Delonix regia |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Pyrrolidinone | - |
Phaseolus vulgaris | |
3,4-Dehydroproline | - |
Delonix regia | |
3,4-Dehydroproline | - |
Phaseolus vulgaris | |
3-pyrroline | - |
Delonix regia | |
3-pyrroline | - |
Phaseolus vulgaris | |
4-Methylene-DL-proline | weak | Delonix regia | |
cis(exo)-3,4-Methano-L-proline | - |
Delonix regia | |
cis(exo)-3,4-Methano-L-proline | - |
Phaseolus vulgaris | |
cis-3-hydroxy-L-proline | weak | Delonix regia | |
cis-3-hydroxy-L-proline | weak | Phaseolus vulgaris | |
CsCl | concentration required to inhibit ATP-diphosphate exchange by 50%: above 2 M | Beta vulgaris | |
CsCl | concentration required to inhibit ATP-diphosphate exchange by 50%: 0.5 M | Delonix regia | |
CsCl | concentration required to inhibit ATP-diphosphate exchange by 50%: 0.51 M | Parkinsonia aculeata | |
CsCl | concentration required to inhibit ATP-diphosphate exchange by 50%: 1.7 M | Phaseolus vulgaris | |
DL-Proline amide | - |
Phaseolus vulgaris | |
KCl | concentration required to inhibit ATP-diphosphate exchange by 50%: above 2 M | Beta vulgaris | |
KCl | concentration required to inhibit ATP-diphosphate exchange by 50%: 0.37 M | Delonix regia | |
KCl | concentration required to inhibit ATP-diphosphate exchange by 50%: 0.4 M | Parkinsonia aculeata | |
KCl | concentration required to inhibit ATP-diphosphate exchange by 50%: 2.1 M | Phaseolus vulgaris | |
L-azetidine-2-carboxylic acid | - |
Delonix regia | |
L-azetidine-2-carboxylic acid | - |
Phaseolus vulgaris | |
L-thiazolidine-4-carboxylic acid | - |
Delonix regia | |
L-thiazolidine-4-carboxylic acid | - |
Phaseolus vulgaris | |
LiCl | concentration required to inhibit ATP-diphosphate exchange by 50%: 1.2 M | Beta vulgaris | |
LiCl | concentration required to inhibit ATP-diphosphate exchange by 50%: 0.25 M | Delonix regia | |
LiCl | concentration required to inhibit ATP-diphosphate exchange by 50%: 0.23 M | Parkinsonia aculeata | |
LiCl | concentration required to inhibit ATP-diphosphate exchange by 50%: 1.2 M | Phaseolus vulgaris | |
N-ethylglycine | weak | Delonix regia | |
N-Methyl-L-alanine | weak | Phaseolus vulgaris | |
N-methylglycine | weak | Delonix regia | |
N-methylglycine | - |
Phaseolus vulgaris | |
NaCl | concentration required to inhibit ATP-diphosphate exchange by 50%: 1.5 M | Beta vulgaris | |
NaCl | concentration required to inhibit ATP-diphosphate exchange by 50%: 0.3 M | Delonix regia | |
NaCl | concentration required to inhibit ATP-diphosphate exchange by 50%: 0.27 M | Parkinsonia aculeata | |
NaCl | concentration required to inhibit ATP-diphosphate exchange by 50%: 1.43 M | Phaseolus vulgaris | |
NH4Cl | concentration required to inhibit ATP-diphosphate exchange by 50%: 1.0 M | Beta vulgaris | |
NH4Cl | concentration required to inhibit ATP-diphosphate exchange by 50%: 0.35 M | Delonix regia | |
NH4Cl | concentration required to inhibit ATP-diphosphate exchange by 50%: 0.38 M | Parkinsonia aculeata | |
NH4Cl | concentration required to inhibit ATP-diphosphate exchange by 50%: 1.1 M | Phaseolus vulgaris | |
Pyrrole | - |
Delonix regia | |
Pyrrole | - |
Phaseolus vulgaris | |
Pyrrolidine | - |
Delonix regia | |
Pyrrolidine | - |
Phaseolus vulgaris | |
tetrahydrofuran | - |
Delonix regia | |
tetrahydrofuran | - |
Phaseolus vulgaris | |
Tetrahydrothiophen | - |
Delonix regia | |
Tetrahydrothiophen | - |
Phaseolus vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.137 | - |
L-Pro | ATP-diphosphate exchange | Phaseolus vulgaris | |
0.182 | - |
L-Pro | ATP-diphosphate exchange | Delonix regia | |
0.28 | - |
3,4-dehydro-DL-proline | ATP-diphosphate exchange | Phaseolus vulgaris | |
0.29 | - |
L-Pro | ATP-diphosphate exchange | Ranunculus bulbosus | |
0.364 | - |
3,4-dehydro-DL-proline | ATP-diphosphate exchange | Ranunculus bulbosus | |
0.43 | - |
L-Pro | ATP-diphosphate exchange | Parkinsonia aculeata | |
0.45 | - |
L-Pro | ATP-diphosphate exchange | Beta vulgaris | |
0.45 | - |
L-Pro | ATP-diphosphate exchange | Convallaria majalis | |
0.5 | - |
3,4-dehydro-DL-proline | ATP-diphosphate exchange | Beta vulgaris | |
0.625 | - |
L-Pro | ATP-diphosphate exchange | Hemerocallis fulva | |
0.74 | - |
3,4-dehydro-DL-proline | ATP-diphosphate exchange | Hemerocallis fulva | |
0.78 | - |
3,4-dehydro-DL-proline | ATP-diphosphate exchange | Delonix regia | |
1.4 | - |
3-thiaproline | 3,4-dehydro-DL-proline, , ATP-diphosphate exchange | Convallaria majalis | |
1.43 | - |
L-azetidine-2-carboxylic acid | ATP-diphosphate exchange | Phaseolus vulgaris | |
2 | - |
L-azetidine-2-carboxylic acid | ATP-diphosphate exchange | Ranunculus bulbosus | |
2 | 3 | cis(exo)-3,4-Methano-L-proline | ATP-diphosphate exchange | Convallaria majalis | |
2.2 | - |
3,4-dehydro-DL-proline | ATP-diphosphate exchange | Beta vulgaris | |
2.2 | - |
3,4-dehydro-DL-proline | ATP-diphosphate exchange, L-azetidine-2-carboxylic acid | Parkinsonia aculeata | |
4.6 | - |
cis(exo)-3,4-Methano-L-proline | ATP-diphosphate exchange | Delonix regia | |
5.3 | - |
L-azetidine-2-carboxylic acid | ATP-diphosphate exchange | Hemerocallis fulva | |
7.1 | - |
cis(exo)-3,4-Methano-L-proline | ATP-diphosphate exchange | Parkinsonia aculeata | |
20 | 50 | L-thiazolidine-4-carboxylic acid | ATP-diphosphate exchange | Delonix regia | |
20 | - |
thiazolidine-4-carboxylic acid | ATP-diphosphate exchange | Phaseolus vulgaris | |
45 | - |
N-methylglycine | ATP-diphosphate exchange | Parkinsonia aculeata | |
67 | - |
N-methylglycine | ATP-diphosphate exchange | Phaseolus vulgaris | |
68 | - |
N-Methyl-L-alanine | ATP-diphosphate exchange | Phaseolus vulgaris | |
100 | - |
N-methylglycine | ATP-diphosphate exchange | Hemerocallis fulva | |
140 | - |
N-methylglycine | ATP-diphosphate exchange | Ranunculus bulbosus | |
300 | - |
N-methylglycine | approximate value, , ATP-diphosphate exchange | Delonix regia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Beta vulgaris | - |
- |
- |
Convallaria majalis | - |
- |
- |
Delonix regia | - |
- |
- |
Hemerocallis fulva | - |
- |
- |
Parkinsonia aculeata | - |
- |
- |
Phaseolus vulgaris | - |
- |
- |
Polygonatum multiflorum | - |
- |
- |
Ranunculus bulbosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Phaseolus vulgaris |
- |
Delonix regia |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.101 | - |
- |
Delonix regia |
1.655 | - |
- |
Delonix regia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3,4-dehydro-DL-proline + tRNAPro | - |
Phaseolus vulgaris | ? | - |
? | |
ATP + 3,4-dehydro-DL-proline + tRNAPro | - |
Delonix regia | ? | - |
? | |
ATP + 3,4-dehydro-DL-proline + tRNAPro | - |
Hemerocallis fulva | ? | - |
? | |
ATP + 3,4-dehydro-DL-proline + tRNAPro | - |
Ranunculus bulbosus | ? | - |
? | |
ATP + 3,4-dehydro-DL-proline + tRNAPro | - |
Beta vulgaris | ? | - |
? | |
ATP + 3,4-dehydro-DL-proline + tRNAPro | - |
Parkinsonia aculeata | ? | - |
? | |
ATP + 3-thiaproline + tRNAPro | - |
Convallaria majalis | ? | - |
? | |
ATP + cis(exo)-3,4-methano-L-proline + tRNAPro | - |
Delonix regia | ? | - |
? | |
ATP + cis(exo)-3,4-methano-L-proline + tRNAPro | - |
Parkinsonia aculeata | ? | - |
? | |
ATP + cis(exo)-3,4-methano-L-proline + tRNAPro | - |
Convallaria majalis | ? | - |
? | |
ATP + L-azetidine-2-carboxylic acid + tRNAPro | - |
Phaseolus vulgaris | ? | - |
? | |
ATP + L-azetidine-2-carboxylic acid + tRNAPro | - |
Hemerocallis fulva | ? | - |
? | |
ATP + L-azetidine-2-carboxylic acid + tRNAPro | - |
Ranunculus bulbosus | ? | - |
? | |
ATP + L-proline + tRNAPro | - |
Polygonatum multiflorum | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | N-methylglycine can replace proline in ATP-diphosphate exchange | Phaseolus vulgaris | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | N-methylglycine can replace proline in ATP-diphosphate exchange | Delonix regia | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | N-methylglycine can replace proline in ATP-diphosphate exchange | Hemerocallis fulva | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | N-methylglycine can replace proline in ATP-diphosphate exchange | Ranunculus bulbosus | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | N-methylglycine can replace proline in ATP-diphosphate exchange | Beta vulgaris | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | N-methylglycine can replace proline in ATP-diphosphate exchange | Parkinsonia aculeata | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | N-methylglycine can replace proline in ATP-diphosphate exchange | Convallaria majalis | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | L-azetidine-2-carboxylic acid can replace proline in ATP-diphosphate exchange (not) | Phaseolus vulgaris | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | L-azetidine-2-carboxylic acid can replace proline in ATP-diphosphate exchange (not) | Hemerocallis fulva | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | L-azetidine-2-carboxylic acid can replace proline in ATP-diphosphate exchange (not) | Ranunculus bulbosus | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | L-azetidine-2-carboxylic acid can replace proline in ATP-diphosphate exchange (not) | Beta vulgaris | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | cis(exo)-3,4-methano-L-proline can replace proline on ATP-diphosphate exchange | Delonix regia | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | cis(exo)-3,4-methano-L-proline can replace proline on ATP-diphosphate exchange | Parkinsonia aculeata | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | cis(exo)-3,4-methano-L-proline can replace proline on ATP-diphosphate exchange | Convallaria majalis | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | 3,4-dehydro-DL-proline can replace proline in ATP-diphosphate exchange | Phaseolus vulgaris | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | 3,4-dehydro-DL-proline can replace proline in ATP-diphosphate exchange | Delonix regia | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | 3,4-dehydro-DL-proline can replace proline in ATP-diphosphate exchange | Hemerocallis fulva | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | 3,4-dehydro-DL-proline can replace proline in ATP-diphosphate exchange | Ranunculus bulbosus | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | 3,4-dehydro-DL-proline can replace proline in ATP-diphosphate exchange | Beta vulgaris | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | 3,4-dehydro-DL-proline can replace proline in ATP-diphosphate exchange | Parkinsonia aculeata | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | 3,4-dehydro-DL-proline can replace proline in ATP-diphosphate exchange | Convallaria majalis | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | L-thiazolidine-4-carboxylic acid can replace proline in ATP-diphosphate exchange | Phaseolus vulgaris | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | L-thiazolidine-4-carboxylic acid can replace proline in ATP-diphosphate exchange | Delonix regia | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | L-thiazolidine-4-carboxylic acid can replace proline in ATP-diphosphate exchange | Ranunculus bulbosus | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | L-thiazolidine-4-carboxylic acid can replace proline in ATP-diphosphate exchange | Beta vulgaris | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | L-thiazolidine-4-carboxylic acid can replace proline in ATP-diphosphate exchange | Parkinsonia aculeata | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-thiazolidine-4-carboxylic acid + tRNAPro | - |
Delonix regia | ? | - |
? | |
ATP + N-methyl-L-alanine + tRNAPro | - |
Phaseolus vulgaris | ? | - |
? | |
ATP + N-methylglycine + tRNAPro | - |
Phaseolus vulgaris | ? | - |
? | |
ATP + N-methylglycine + tRNAPro | - |
Delonix regia | ? | - |
? | |
ATP + N-methylglycine + tRNAPro | - |
Hemerocallis fulva | ? | - |
? | |
ATP + N-methylglycine + tRNAPro | - |
Ranunculus bulbosus | ? | - |
? | |
ATP + N-methylglycine + tRNAPro | - |
Parkinsonia aculeata | ? | - |
? | |
ATP + thiazolidine-4-carboxylic acid + tRNAPro | - |
Phaseolus vulgaris | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
50% loss of ATP-diphosphate exchange activity after 5 min, without stabilizing reagent | Delonix regia |
25 | - |
50% loss of ATP-diphosphate exchange activity after 5 min, without stabilizing reagent | Parkinsonia aculeata |
51 | - |
50% loss of ATP-diphosphate exchange activity after 5 min, without stabilizing reagent | Ranunculus bulbosus |
55 | - |
50% loss of ATP-diphosphate exchange activity after 5 min, in presence of 0.002 mM ATP | Parkinsonia aculeata |
56 | - |
50% loss of ATP-diphosphate exchange activity after 5 min, in presence of 0.002 mM ATP | Delonix regia |
58 | - |
50% loss of ATP-diphosphate exchange activity after 5 min, in presence of 0.02 mM Pro | Parkinsonia aculeata |
59 | - |
50% loss of ATP-diphosphate exchange activity after 5 min, in presence of 0.02 mM Pro | Delonix regia |
65 | - |
50% loss of ATP-diphosphate exchange activity after 5 min, without stabilizing reagent | Phaseolus vulgaris |
67 | - |
50% loss of ATP-diphosphate exchange activity after 5 min, in presence of 0.002 mM ATP + 0.02 mM Pro | Delonix regia |
67 | - |
50% loss of ATP-diphosphate exchange activity after 5 min, in presence of 0.002 mM ATP + 0.02 mM Pro | Parkinsonia aculeata |
73 | - |
50% loss of ATP-diphosphate exchange activity after 5 min, in presence of 0.002 mM ATP | Phaseolus vulgaris |
78 | - |
50% loss of ATP-diphosphate exchange activity after 5 min, in presence of 0.002 mM ATP + 0.02 mM Pro | Phaseolus vulgaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 7.8 | ATP-diphosphate exchange | Phaseolus vulgaris |
7.5 | 7.8 | ATP-diphosphate exchange | Ranunculus bulbosus |
7.6 | 7.9 | ATP-diphosphate exchange | Hemerocallis fulva |
7.9 | 8.3 | ATP-diphosphate exchange | Beta vulgaris |
8.4 | 8.7 | ATP-diphosphate exchange | Convallaria majalis |
8.4 | 8.8 | ATP-diphosphate exchange | Polygonatum multiflorum |
8.8 | 9.2 | ATP-diphosphate exchange | Parkinsonia aculeata |
9 | 9.4 | ATP-diphosphate exchange | Delonix regia |