Literature summary for 6.1.1.14 extracted from

Cader, M.Z.; Ren, J.; James, P.A.; Bird, L.E.; Talbot, K.; Stammers, D.K.
Crystal structure of human wildtype and S581L-mutant glycyl-tRNA synthetase, an enzyme underlying distal spinal muscular atrophy (2007), FEBS Lett., 581, 2959-2964.

Search for more literature for 6.1.1.14

Cloned(Commentary)

Cloned (Comment)	Organism
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified His-tagged wild-type and S581L mutant enzymes, sitting drop vapour diffusion nanocrystallization method, mutant S581L GlyRS from 20% PEG 3350, 0.2 M Na2SO4, 0.1 M Bis-Tris propane, pH 6.5, wild-type GlyRS from 20% PEG 3350, 0.2M sodium bromide, 0.1 M bis-Tris propane pH 8.5, X-ray diffraction structure determination and anaylsis at 2.8A and 3.1 A resolution, respectively	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
S581L	the naturally occuring dominant mutation to reduced aminoacylation activity and to motor nerve degeneration	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + glycine + tRNAGly	Homo sapiens	enzyme mutations with reduced enzyme activity cause distal spinal muscular atrophy type V or Charcot-Marie-Tooth type 2D, i.e. dSMAV/CMT2D	AMP + diphosphate + glycyl-tRNAGly	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	gene GARS	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + glycine + tRNAGly	enzyme mutations with reduced enzyme activity cause distal spinal muscular atrophy type V or Charcot-Marie-Tooth type 2D, i.e. dSMAV/CMT2D	Homo sapiens	AMP + diphosphate + glycyl-tRNAGly	-	?
ATP + glycine + tRNAGly	tRNA binding site structure, and wild-type and S581L mutant active site structures, overview	Homo sapiens	AMP + diphosphate + glycyl-tRNAGly	-	?

Synonyms

Synonyms	Comment	Organism
Glycyl-tRNA synthetase	-	Homo sapiens
GlyRS	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens

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Release 2023.1 (January 2023)