Cloned (Comment) | Organism |
---|---|
gene GARS, lacking the mitochondrial-specific coding region, expression as C-terminally His6-tagged cytosolic enzyme in Escherichia coli strain BL21(DE3) | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant soluble enzyme, hanging drop vapour diffusion method, 0.001 ml of 8 mg/ml protein in 10 mM HEPES, pH 7.0, with 20 mM NaCl is mixed with 0.001 ml of reservoir solution containing 10% PEG 6000, 0.1 HEPES pH 6.5, 0.01 Tris-HCl, pH 8.5, 0.5 NaCl and 0.1 magnesium acetate at room temperature, 2 days, X-ray diffraction structure determination and analysis at 3.0 A resolution | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glycine + tRNAGly | Homo sapiens | GlyRS allelic variants are causally associated with the Charcot-Marie-Tooth disease, the most common genetic disorder of the peripheral nervous system | AMP + diphosphate + glycyl-tRNAGly | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P41250 | gene GARS | - |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His6-tagged cytosolic enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glycine + tRNAGly | - |
Homo sapiens | AMP + diphosphate + glycyl-tRNAGly | - |
? | |
ATP + glycine + tRNAGly | GlyRS allelic variants are causally associated with the Charcot-Marie-Tooth disease, the most common genetic disorder of the peripheral nervous system | Homo sapiens | AMP + diphosphate + glycyl-tRNAGly | - |
? | |
additional information | structure-function analysis | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Glycyl-tRNA synthetase | - |
Homo sapiens |
GlyRS | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |