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Literature summary for 6.1.1.14 extracted from

  • Uwer, U.; Willmitzer, L.; Altmann, T.
    Inactivation of a glycyl-tRNA synthetase leads to an arrest in plant embryo development (1998), Plant Cell, 10, 1277-1294.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination and analysis of full-length edd1 wild-type gene, encoding the enzyme, gene contains a chloroplast targeting sequence, determination of the promoter, functional complementation of enzyme-deficient Arabidopsis plants thereby generation of transgenic plants Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information direction of a marker protein into isolated pea chloroplasts by the N-terminal domain of the enzyme, construction of the embryo-defective development mutant edd1 by usage of transposons as insertional mutagens, the reversible mutation leads to arrest of embryo growth and is lethal, generation of transgenic plants Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Arabidopsis thaliana 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
117000
-
x * 117000, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + glycine + tRNAGly Arabidopsis thaliana
-
AMP + diphosphate + glycyl-tRNAGly
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q8L785
-
-

Source Tissue

Source Tissue Comment Organism Textmining
embryo in the early-globular stage Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + glycine + tRNAGly
-
Arabidopsis thaliana AMP + diphosphate + glycyl-tRNAGly
-
?

Subunits

Subunits Comment Organism
? x * 117000, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
Glycine--tRNA ligase
-
Arabidopsis thaliana
Glycyl translase
-
Arabidopsis thaliana
Glycyl-transfer ribonucleate synthetase
-
Arabidopsis thaliana
Glycyl-transfer ribonucleic acid synthetase
-
Arabidopsis thaliana
Glycyl-transfer RNA synthetase
-
Arabidopsis thaliana
Glycyl-tRNA synthetase
-
Arabidopsis thaliana
GlyRS
-
Arabidopsis thaliana
Synthetase, glycyl-transfer ribonucleate
-
Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP
-
Arabidopsis thaliana