Literature summary for 6.1.1.14 extracted from

Dignam, J.D.; Nada, S.; Chaires, J.B.
Thermodynamic characterization of the binding of nucleotides to glycyl-tRNA synthetase (2003), Biochemistry, 42, 5333-5340.

Search for more literature for 6.1.1.14

General Stability

General Stability	Organism
ATP and nonsubstrate ATP analogues render the enzyme more resistant to digestion by several proteases, e.g. thrombin, Arg-C, and chymotrypsin	Bombyx mori

Inhibitors

Inhibitors	Comment	Organism	Structure
5'-adenylylimidodiphosphate	i.e. AMPPNP, a nonsubstrate ATP analogue, competitive inhibition	Bombyx mori
adenylylmethylenediphosphate	i.e. AMPPCP, a nonsubstrate ATP analogue, competitive inhibition	Bombyx mori
alpha,beta-methyleneadenosine 5'-diphosphate	i.e. AMPCPP, a nonsubstrate ATP analogue, competitive inhibition	Bombyx mori
AMP	competitive inhibition	Bombyx mori
glycyl sulfamoyl adenosine	-	Bombyx mori

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	thermodynamic parameters of substrate and inhibitor binding	Bombyx mori
0.013	-	ATP	-	Bombyx mori

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + glycine + tRNAGly	Bombyx mori	-	AMP + diphosphate + glycyl-tRNAGly	-	?

Organism

Organism	UniProt	Comment	Textmining
Bombyx mori	-	recombinant purified enzyme, expressed in Escherichia coli	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + glycine + tRNAGly	-	Bombyx mori	AMP + diphosphate + glycyl-tRNAGly	-	?

Synonyms

Synonyms	Comment	Organism
Glycine--tRNA ligase	-	Bombyx mori
Glycyl translase	-	Bombyx mori
Glycyl-transfer ribonucleate synthetase	-	Bombyx mori
Glycyl-transfer ribonucleic acid synthetase	-	Bombyx mori
Glycyl-transfer RNA synthetase	-	Bombyx mori
Glycyl-tRNA synthetase	-	Bombyx mori
GlyRS	-	Bombyx mori
Synthetase, glycyl-transfer ribonucleate	-	Bombyx mori

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Bombyx mori

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0008	-	adenylylmethylenediphosphate	-	Bombyx mori
0.02	-	5'-adenylylimidodiphosphate	-	Bombyx mori
0.06	-	alpha,beta-methyleneadenosine 5'-diphosphate	-	Bombyx mori
0.08	-	AMP	-	Bombyx mori

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Release 2023.1 (January 2023)