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Literature summary for 6.1.1.14 extracted from
- Glycyl transfer ribonucleic acid synthetase from Escherichia coli. Purification, properties, and substrate binding (1974), Biochemistry, 13, 1338-1348.
General Stability
| General Stability | Organism |
|---|---|
| aminoethyl-AMP protects from heat inactivation | Escherichia coli |
| glycerol required for stabilization | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Aminoethyl-AMP | - |
Escherichia coli |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0002 | - |
tRNAGly | glycyl-tRNA formation | Escherichia coli | |
| 0.014 | - |
ATP | ATP, , ATP-diphosphate exchange, without tRNA | Escherichia coli | |
| 0.03 | - |
Gly | ATP-diphosphate exchange, without tRNA | Escherichia coli | |
| 0.04 | - |
ATP | ATP-diphosphate exchange in presence of tRNA | Escherichia coli | |
| 0.042 | - |
ATP | glycyl-tRNA formation | Escherichia coli | |
| 0.14 | - |
Gly | ATP-diphosphate exchange in presence of tRNA | Escherichia coli | |
| 0.16 | - |
Gly | glycyl-tRNA formation | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
2 * 33000 (alpha) + 2 * 80000 (beta), SDS-PAGE | Escherichia coli |
| 80000 | - |
2 * 33000 (alpha) + 2 * 80000 (beta), SDS-PAGE | Escherichia coli |
| 227000 | - |
sedimentation equilibrium ultracentrifugation | Escherichia coli |
| 240000 | - |
PAGE | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.7 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, enzyme concentration 0.5 mg/ml, 40% glycerol, 30% loss of activity after 8 months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + glycine + tRNAGly | binds two molecules of glycyl-AMP per tetramer | Escherichia coli | AMP + diphosphate + glycyl-tRNAGly | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 2 * 33000 (alpha) + 2 * 80000 (beta), SDS-PAGE | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
80% loss of activity after 1 min in absence of substrates, 60% loss of activity after 10 min in presence of aminoethyl-AMP and Mg2+ | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Escherichia coli |
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