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Literature summary for 6.1.1.12 extracted from

  • Fuengfuloy, P.; Chuawong, P.; Suebka, S.; Wattana-Amorn, P.; Williams, C.; Crump, M.P.; Songsiriritthigul, C.
    Overproduction of the N-terminal anticodon-binding domain of the non-discriminating aspartyl-tRNA synthetase from Helicobacter pylori for crystallization and NMR measurements (2013), Protein Expr. Purif., 89, 25-32.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Helicobacter pylori

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, in 30% (w/v) PEG 4000, 100 mM ammonium sulfate, 100 mM sodium citrate at pH 5.6 Helicobacter pylori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12870
-
1 * 12870, calculated from amino acid sequence Helicobacter pylori
15500
-
gel filtration Helicobacter pylori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-aspartate + tRNAAsn Helicobacter pylori
-
AMP + diphosphate + L-aspartyl-tRNAAsn
-
?
ATP + L-aspartate + tRNAAsp Helicobacter pylori
-
AMP + diphosphate + L-aspartyl-tRNAAsp
-
?

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Hiprep 16/60 Sephacryl S-200 gel filtration Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate + tRNAAsn
-
Helicobacter pylori AMP + diphosphate + L-aspartyl-tRNAAsn
-
?
ATP + L-aspartate + tRNAAsp
-
Helicobacter pylori AMP + diphosphate + L-aspartyl-tRNAAsp
-
?

Subunits

Subunits Comment Organism
monomer 1 * 12870, calculated from amino acid sequence Helicobacter pylori

Synonyms

Synonyms Comment Organism
AsnRS
-
Helicobacter pylori
Aspartyl-tRNA synthetase
-
Helicobacter pylori

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 42 the enzyme is stable up to 42°C Helicobacter pylori

Cofactor

Cofactor Comment Organism Structure
ATP
-
Helicobacter pylori