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Literature summary for 6.1.1.12 extracted from
- Aspartyl-tRNA synthetase of the hyperthermophilic archaeon Pyrococcus sp. KOD1 has a chimerical structure of eukaryotic and bacterial enzymes (1995), Gene, 164, 153-156.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Thermococcus kodakarensis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
x * 50000, SDS-PAGE | Thermococcus kodakarensis |
| 50000 | - |
x * 50000, calculated from sequence | Thermococcus kodakarensis |
| 50893 | - |
x * 50893, calculated from sequence | Thermococcus kodakarensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermococcus kodakarensis | Q52428 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermococcus kodakarensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.11 | - |
65°C, pH is not specified in the publication | Thermococcus kodakarensis |
| 0.11 | - |
65°C, pH not specified in the publication | Thermococcus kodakarensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-aspartate + tRNAAsp | - |
Thermococcus kodakarensis | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, SDS-PAGE | Thermococcus kodakarensis |
| ? | x * 50893, calculated from sequence | Thermococcus kodakarensis |
| ? | x * 50000, calculated from sequence | Thermococcus kodakarensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Aspartyl-tRNA synthetase | - |
Thermococcus kodakarensis |
| AspRS | - |
Thermococcus kodakarensis |
| TK0492 | locus name | Thermococcus kodakarensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
assay at | Thermococcus kodakarensis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Thermococcus kodakarensis | calculated from sequence | - |
5.23 |
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