Protein Variants | Comment | Organism |
---|---|---|
Q199E | site-directed mutagenesis and molecular dynamics simulation of the mutation, reduction in binding free energy in the simulation in agreement with experiment, overview | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
succinate | 50% inhibition at 210 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, thermodynamics of wild-type enzyme and mutant Q199E | Escherichia coli | |
0.32 | - |
L-Asp | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsp | Escherichia coli | - |
AMP + diphosphate + aspartyl-tRNAAsp | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P21889 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-aspartate + tRNAAsp = AMP + diphosphate + L-aspartyl-tRNAAsp | active site structure, AspRS substrate recognition and chiral specificity of wild-type enzyme and mutant Q199E, substrate binding in regular versus inverted orientation, overview | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-aspartate + tRNAAsp | aspartyl-tRNA synthetase can misacylate tRNAAsp with D-aspartate instead of its usual substrate, L-Asp, substrate specificity and molecular dynamics simulations, overview | Escherichia coli | AMP + diphosphate + D-aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAsp | aspartyl-tRNA synthetase can misacylate tRNAAsp with D-aspartate instead of its usual substrate, L-Asp, substrate specificity and molecular dynamics simulations, overview | Escherichia coli | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAsp | - |
Escherichia coli | AMP + diphosphate + aspartyl-tRNAAsp | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Aspartyl-tRNA synthetase | - |
Escherichia coli |
AspRS | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |