Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.12 extracted from

  • Brevet, A.; Chen, J.; Commans, S.; Lazennec, C.; Blanquet, S.; Plateau, P.
    Anticodon Recognition in Evolution: Switching tRNA specificity of an aminoacyl-tRNA synthetase by site-directed (2003), J. Biol. Chem., 278, 30927-30935.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information transplantation of the L45 loop of Escherichia coli AspRS and two residues flanking the loop inside the Escherichia coli lysyl-tRNALys ligase sequence causes a loss of lysyl-tRNALys ligase capacity to aminoacylate tRNALys. The chimeric enzyme acquires the capacity to charge tRNAAsp with lysine Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
tRNAAsp pH 7.8, 28°C Escherichia coli
1.3
-
tRNAAspA73 pH 7.8, 28°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Asp + tRNAAsp low reaction with mutant tRNAAsp with A instead of G at position 73 (tRNAAspA73) Escherichia coli AMP + diphosphate + aspartyl-tRNAAsp
-
?
ATP + L-aspartate + tRNAAspA73
-
Escherichia coli AMP + diphosphate + L-aspartyl-tRNAAspA73
-
?
additional information no transfer of lysine on tRNAAsp Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
AspRS
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0029
-
tRNAAspA73 pH 7.8, 28°C Escherichia coli
0.55
-
tRNAAsp pH 7.8, 28°C Escherichia coli