Protein Variants | Comment | Organism |
---|---|---|
additional information | transplantation of the L45 loop of Escherichia coli AspRS and two residues flanking the loop inside the Escherichia coli lysyl-tRNALys ligase sequence causes a loss of lysyl-tRNALys ligase capacity to aminoacylate tRNALys. The chimeric enzyme acquires the capacity to charge tRNAAsp with lysine | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
tRNAAsp | pH 7.8, 28°C | Escherichia coli | |
1.3 | - |
tRNAAspA73 | pH 7.8, 28°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Asp + tRNAAsp | low reaction with mutant tRNAAsp with A instead of G at position 73 (tRNAAspA73) | Escherichia coli | AMP + diphosphate + aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAspA73 | - |
Escherichia coli | AMP + diphosphate + L-aspartyl-tRNAAspA73 | - |
? | |
additional information | no transfer of lysine on tRNAAsp | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AspRS | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0029 | - |
tRNAAspA73 | pH 7.8, 28°C | Escherichia coli | |
0.55 | - |
tRNAAsp | pH 7.8, 28°C | Escherichia coli |