Cloned (Comment) | Organism |
---|---|
overexpression of wild-type and mutants | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
D210A | central core binding mutant, increased acylation activity, increased dissociation constant | Saccharomyces cerevisiae |
E188A | anticodon loop binding mutant, slightly reduced acylation activity, increased dissociation constant | Saccharomyces cerevisiae |
E188A/S239A | anticodon-G73 binding mutant, reduced acylation activity, highly increased dissociation constant | Saccharomyces cerevisiae |
E188A/T331A | anticodon-G73 binding mutant, highly reduced acylation activity, highly increased dissociation constant | Saccharomyces cerevisiae |
E202A | central core binding mutant, slightly increased acylation activity, increased dissociation constant | Saccharomyces cerevisiae |
E327A | acceptor arm binding mutant, reduced acylation activity | Saccharomyces cerevisiae |
F127A | anticodon loop binding mutant, increased acylation activity, increased dissociation constant | Saccharomyces cerevisiae |
F127A/D210A | anticodon binding mutant, reduced acylation activity, highly increased dissociation constant | Saccharomyces cerevisiae |
F127A/E188A | anticodon binding mutant, reduced acylation activity, highly increased dissociation constant | Saccharomyces cerevisiae |
F127A/S239A | anticodon-G73 binding mutant, reduced acylation activity, highly increased dissociation constant | Saccharomyces cerevisiae |
F127A/T331A | anticodon-G73 binding mutant, highly reduced acylation activity, highly increased dissociation constant | Saccharomyces cerevisiae |
F304A | terminal A binding mutant, reduced acylation activity | Saccharomyces cerevisiae |
H334A | acceptor arm binding mutant, highly reduced acylation activity, increased dissociation constant | Saccharomyces cerevisiae |
K142A | anticodon loop binding mutant, increased acylation activity, increased dissociation constant | Saccharomyces cerevisiae |
K142A/E188A | anticodon binding mutant, reduced acylation activity, highly increased dissociation constant | Saccharomyces cerevisiae |
K155A | central core binding mutant, increased acylation activity, increased dissociation constant | Saccharomyces cerevisiae |
K180A | anticodon loop binding mutant, slightly increased acylation activity, increased dissociation constant | Saccharomyces cerevisiae |
K293A | acceptor arm binding mutant, highly increased acylation activity, increased dissociation constant | Saccharomyces cerevisiae |
K428A | acceptor arm binding mutant, increased acylation activity, increased dissociation constant | Saccharomyces cerevisiae |
K553A | acceptor arm binding mutant, acylation activity similar to wild-type, increased dissociation constant | Saccharomyces cerevisiae |
additional information | cooperativity analysis of the G73 and anticodon binding interactions, overview, effect of mutations on cell growth | Saccharomyces cerevisiae |
N117A | anticodon loop binding mutant, increased acylation activity, increased dissociation constant | Saccharomyces cerevisiae |
N227A | central core binding mutant, slightly increased acylation activity, increased dissociation constant | Saccharomyces cerevisiae |
N328A | acceptor arm binding mutant, reduced acylation activity, increased dissociation constant | Saccharomyces cerevisiae |
N328A/S329A/T331A | G73 binding mutant, highly reduced acylation activity, increased dissociation constant | Saccharomyces cerevisiae |
Q121A | anticodon loop binding mutant, increased acylation activity, increased dissociation constant | Saccharomyces cerevisiae |
Q138A | anticodon loop binding mutant, slightly increased acylation activity, increased dissociation constant | Saccharomyces cerevisiae |
Q138A/E188A | anticodon binding mutant, reduced acylation activity, highly increased dissociation constant | Saccharomyces cerevisiae |
Q138A/R119A | anticodon binding mutant, reduced acylation activity, highly increased dissociation constant | Saccharomyces cerevisiae |
Q300A | terminal A binding mutant, reduced acylation activity | Saccharomyces cerevisiae |
R119A | anticodon loop binding mutant, increased acylation activity, increased dissociation constant | Saccharomyces cerevisiae |
R119A/E188A | anticodon binding mutant, highly reduced acylation activity, highly increased dissociation constant | Saccharomyces cerevisiae |
S181A | anticodon loop binding mutant, increased acylation activity, increased dissociation constant | Saccharomyces cerevisiae |
S280A | terminal A binding mutant, reduced acylation activity | Saccharomyces cerevisiae |
S301A | terminal A binding mutant, reduced acylation activity, slightly increased dissociation constant | Saccharomyces cerevisiae |
S329A | acceptor arm binding mutant, increased acylation activity, increased dissociation constant | Saccharomyces cerevisiae |
S423A | acceptor arm binding mutant, reduced acylation activity | Saccharomyces cerevisiae |
T124A | anticodon loop binding mutant, slightly reduced acylation activity, increased dissociation constant | Saccharomyces cerevisiae |
T230A | central core binding mutant, increased acylation activity, increased dissociation constant | Saccharomyces cerevisiae |
T331A | acceptor arm binding mutant, reduced acylation activity, increased dissociation constant | Saccharomyces cerevisiae |
T424A | acceptor arm binding mutant, acylation activity similar to the wild-type, increased dissociation constant | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsp | Saccharomyces cerevisiae | - |
AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
recombinant purified enzyme | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutants | Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-aspartate + tRNAAsp = AMP + diphosphate + L-aspartyl-tRNAAsp | mechanism | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsp | - |
Saccharomyces cerevisiae | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Aspartic acid translase | - |
Saccharomyces cerevisiae |
Aspartyl ribonucleate synthetase | - |
Saccharomyces cerevisiae |
aspartyl ribonuleic synthetase | - |
Saccharomyces cerevisiae |
Aspartyl-transfer ribonucleic acid synthetase | - |
Saccharomyces cerevisiae |
Aspartyl-transfer RNA synthetase | - |
Saccharomyces cerevisiae |
Aspartyl-tRNA synthetase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.3 | - |
tRNAAsp | wild-type enzyme, acylation activity, pH 7.5, 37°C | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharomyces cerevisiae |