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Literature summary for 6.1.1.12 extracted from

  • Zhu, D.W.; Lorber, B.; Sauter, C.; Ng, J.D.; Benas, P.; Le Grimellec, C.; Giege, R.
    Growth kinetics, diffraction properties and effect of agarose on the stability of a novel crystal form of Thermus thermophilus aspartyl-tRNA synthetase-1 (2001), Acta Crystallogr. Sect. D, 57, 552-558.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Thermus thermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, hanging-drop vapour diffusion method, in presence of PEG 8000, at 293K, growth kinetics and solubility measurements, X-ray diffraction structure determination at 3.1 A resolution Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-aspartate + tRNAAsp Thermus thermophilus
-
AMP + diphosphate + L-aspartyl-tRNAAsp
-
?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
isozyme AspRS1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from overexpressing Escherichia coli Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate + tRNAAsp
-
Thermus thermophilus AMP + diphosphate + L-aspartyl-tRNAAsp
-
?

Synonyms

Synonyms Comment Organism
Aspartic acid translase
-
Thermus thermophilus
Aspartyl ribonucleate synthetase
-
Thermus thermophilus
aspartyl ribonuleic synthetase
-
Thermus thermophilus
Aspartyl-transfer ribonucleic acid synthetase
-
Thermus thermophilus
Aspartyl-transfer RNA synthetase
-
Thermus thermophilus
Aspartyl-tRNA synthetase
-
Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
thermostable isozyme AspRS1 Thermus thermophilus