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Literature summary for 6.1.1.12 extracted from

  • Tumbula-Hansen, D.; Feng, L.; Toogood, H.; Stetter, K.O.; Söll, D.
    Evolutionary divergence of the archaeal aspartyl-tRNA synthetases into discriminating and nondiscriminating forms (2002), J. Biol. Chem., 277, 37184-37190.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression as His-tagged protein in Escherichia coli Methanothermobacter thermautotrophicus
expression as His-tagged protein in Escherichia coli Thermococcus kodakarensis
expression as His-tagged protein in Escherichia coli Ferroplasma acidarmanus
expression in Escherichia coli Thermococcus kodakarensis
expression in Escherichia coli Ferroplasma acidarmanus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-aspartate + tRNAAsn Methanothermobacter thermautotrophicus the archaeal AspRS enzyme is nondiscriminating, which means that it forms Asp-tRNAAsp and Asp-tRNAAsn, which is the intermediate in AsntRNAAsn generation by ASp-tRNAAsn amidotransferase, in contrary bacterial enzymes are discriminating ones AMP + diphosphate + L-aspartyl-tRNAAsn
-
?
ATP + L-aspartate + tRNAAsp Methanothermobacter thermautotrophicus
-
AMP + diphosphate + L-aspartyl-tRNAAsp
-
?
ATP + L-aspartate + tRNAAsp Thermococcus kodakarensis
-
AMP + diphosphate + L-aspartyl-tRNAAsp
-
?
ATP + L-aspartate + tRNAAsp Ferroplasma acidarmanus
-
AMP + diphosphate + L-aspartyl-tRNAAsp
-
?
additional information Thermococcus kodakarensis no acylation with L-aspartate of tRNAAsn, enzyme is discriminating, because it forms Asn-tRNAAsn by direct aminoacylation, not via the intermediate of Asp-tRNAAsn, and it contains no Asp-tRNAAsn amidotransferase ?
-
?
additional information Ferroplasma acidarmanus no acylation with L-aspartate of tRNAAsn, enzyme is discriminating, because it forms Asn-tRNAAsn by direct aminoacylation, not via the intermediate of Asp-tRNAAsn, and it contains no Asp-tRNAAsn amidotransferase ?
-
?

Organism

Organism UniProt Comment Textmining
Ferroplasma acidarmanus
-
-
-
Methanothermobacter thermautotrophicus
-
-
-
Thermococcus kodakarensis Q52428
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermococcus kodakarensis
-
Ferroplasma acidarmanus
recombinant His-tagged enzyme from Escherichia coli Methanothermobacter thermautotrophicus
recombinant His-tagged enzyme from Escherichia coli Thermococcus kodakarensis
recombinant His-tagged enzyme from Escherichia coli Ferroplasma acidarmanus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate + tRNAAsn
-
Methanothermobacter thermautotrophicus AMP + diphosphate + L-aspartyl-tRNAAsn
-
?
ATP + L-aspartate + tRNAAsn the archaeal AspRS enzyme is nondiscriminating, which means that it forms Asp-tRNAAsp and Asp-tRNAAsn, which is the intermediate in AsntRNAAsn generation by ASp-tRNAAsn amidotransferase, in contrary bacterial enzymes are discriminating ones Methanothermobacter thermautotrophicus AMP + diphosphate + L-aspartyl-tRNAAsn
-
?
ATP + L-aspartate + tRNAAsp
-
Methanothermobacter thermautotrophicus AMP + diphosphate + L-aspartyl-tRNAAsp
-
?
ATP + L-aspartate + tRNAAsp
-
Thermococcus kodakarensis AMP + diphosphate + L-aspartyl-tRNAAsp
-
?
ATP + L-aspartate + tRNAAsp
-
Ferroplasma acidarmanus AMP + diphosphate + L-aspartyl-tRNAAsp
-
?
ATP + L-aspartate + tRNAAsp no activity with tRNAAsn Thermococcus kodakarensis AMP + diphosphate + L-aspartyl-tRNAAsp
-
?
ATP + L-aspartate + tRNAAsp no activity with tRNAAsn Ferroplasma acidarmanus AMP + diphosphate + L-aspartyl-tRNAAsp
-
?
additional information no acylation with L-aspartate of tRNAAsn Thermococcus kodakarensis ?
-
?
additional information no acylation with L-aspartate of tRNAAsn Ferroplasma acidarmanus ?
-
?
additional information no acylation with L-aspartate of tRNAAsn, enzyme is discriminating, because it forms Asn-tRNAAsn by direct aminoacylation, not via the intermediate of Asp-tRNAAsn, and it contains no Asp-tRNAAsn amidotransferase Thermococcus kodakarensis ?
-
?
additional information no acylation with L-aspartate of tRNAAsn, enzyme is discriminating, because it forms Asn-tRNAAsn by direct aminoacylation, not via the intermediate of Asp-tRNAAsn, and it contains no Asp-tRNAAsn amidotransferase Ferroplasma acidarmanus ?
-
?

Synonyms

Synonyms Comment Organism
Aspartic acid translase
-
Methanothermobacter thermautotrophicus
Aspartic acid translase
-
Thermococcus kodakarensis
Aspartic acid translase
-
Ferroplasma acidarmanus
Aspartyl ribonucleate synthetase
-
Methanothermobacter thermautotrophicus
Aspartyl ribonucleate synthetase
-
Thermococcus kodakarensis
Aspartyl ribonucleate synthetase
-
Ferroplasma acidarmanus
aspartyl ribonuleic synthetase
-
Methanothermobacter thermautotrophicus
aspartyl ribonuleic synthetase
-
Thermococcus kodakarensis
aspartyl ribonuleic synthetase
-
Ferroplasma acidarmanus
Aspartyl-transfer ribonucleic acid synthetase
-
Methanothermobacter thermautotrophicus
Aspartyl-transfer ribonucleic acid synthetase
-
Thermococcus kodakarensis
Aspartyl-transfer ribonucleic acid synthetase
-
Ferroplasma acidarmanus
Aspartyl-transfer RNA synthetase
-
Methanothermobacter thermautotrophicus
Aspartyl-transfer RNA synthetase
-
Thermococcus kodakarensis
Aspartyl-transfer RNA synthetase
-
Ferroplasma acidarmanus
Aspartyl-tRNA synthetase
-
Methanothermobacter thermautotrophicus
Aspartyl-tRNA synthetase
-
Thermococcus kodakarensis
Aspartyl-tRNA synthetase
-
Ferroplasma acidarmanus
AspRS
-
Methanothermobacter thermautotrophicus
discriminating aspartyl-tRNA synthetase
-
Thermococcus kodakarensis
discriminating aspartyl-tRNA synthetase
-
Ferroplasma acidarmanus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Ferroplasma acidarmanus
60
-
-
Thermococcus kodakarensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 60
-
Methanothermobacter thermautotrophicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Thermococcus kodakarensis
6
-
-
Ferroplasma acidarmanus
7
-
assay at Methanothermobacter thermautotrophicus