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Literature summary for 6.1.1.12 extracted from

  • Akesson, B.; Lundvik, L.
    Simultaneous purification and some properties of aspartate:tRNA ligase and seven other amino-acid:tRNA ligases from Escherichia coli (1978), Eur. J. Biochem., 83, 29-36.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
tRNAAsp
-
Escherichia coli
0.05
-
ATP
-
Escherichia coli
0.05
-
Asp
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
2 * 61000, sedimentation equilibrium under denaturing conditions Escherichia coli
64000
-
2 * 64000, PAGE under denaturing conditions Escherichia coli
119000
-
sedimentation equilibrium determination Escherichia coli
132000
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli B / ATCC 11303
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
-80°C, 0.1 M potassium phosphate buffer, about 50% loss of activity after 6 months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate + tRNAAsp
-
Escherichia coli AMP + diphosphate + L-aspartyl-tRNAAsp
-
?
ATP + L-aspartate + tRNAAsp
-
Escherichia coli B / ATCC 11303 AMP + diphosphate + L-aspartyl-tRNAAsp
-
?

Subunits

Subunits Comment Organism
dimer 2 * 61000, sedimentation equilibrium under denaturing conditions Escherichia coli
dimer 2 * 64000, PAGE under denaturing conditions Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
10 min stable, 95% loss of activity after 2 h Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
Tris/cacodylate buffer Escherichia coli